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A response surface approach for the production of natural pigment astaxanthin from green alga Haematococcus pluvialis: Effect of sodium acetate, culture age, and sodium chloride.

IR@CFTRI: CSIR-Central Food Technological Research Institute, Mysore


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Relation	http://ir.cftri.com/2562/

Title	A response surface approach for the production of natural pigment astaxanthin from green alga Haematococcus pluvialis: Effect of sodium acetate, culture age, and sodium chloride.

Creator	Sarada, R. Sila, Bhattacharya Suvendu, Bhattacharya Ravishankar, G. A.

Subject	31 Food Additives 01 Algae

Description	The effect of sodium acetate, sodium chloride and Haematococcus pluvialis culture age on biomass yield, astaxanthin content and astaxanthin production was investigated using response surface methodology in a batch mode of cultivation. An orthogonal experimental design, with three variables (at five coded levels of 1.682, 1, 0, 1 and 1.682) and three response functions, was employed to study the effect of the individual variable on the response functions. The response functions correlated well (r greater than or equal to 0.98, p less than or equal to 0.01) with these variables by second order polynomials consisting of linear, quadratic and interaction terms. The effect of culture age dominated over the sodium acetate and sodium chloride concentrations. Optimum conditions for achieving highastaxanthin content (>1.4%w=w) and astaxanthin production (13 mg=L) were found to be with 3150mM sodium acetate and 0.550.63% (w=v) of sodium chloride.

Date	2002

Type	Article PeerReviewed

Format	application/pdf

Language	en

Identifier	http://ir.cftri.com/2562/1/Food_Biotechnology_2002_16_2_107-120.pdf Sarada, R. and Sila, Bhattacharya and Suvendu, Bhattacharya and Ravishankar, G. A. (2002) A response surface approach for the production of natural pigment astaxanthin from green alga Haematococcus pluvialis: Effect of sodium acetate, culture age, and sodium chloride. Food Biotechnology, 16 (2). pp. 107-120.