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In vitro rooting of Decalepis hamiltonii Wight & Arn., an endangered shrub, by auxins and root-promoting agents

IR@CFTRI: CSIR-Central Food Technological Research Institute, Mysore

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Relation http://ir.cftri.com/2118/
CS-02-01
 
Title In vitro rooting of Decalepis hamiltonii Wight & Arn., an endangered shrub, by auxins and root-promoting agents
 
Creator Obul Reddy, B.
Giridhar, P.
Ravishankar, G. A.
 
Subject 06 Trees And Shrubs
05 Tissue Culture
 
Description In the present study micropropagation of an endangered shrub, Decalepis hamiltonii Wight & Arn., optimization and standardization of efficient methods for rooting of in vitro-derived micro-shoots by using phloroglucinol (PG), activated charcoal and CoCl2 have been achieved. Transfer of in vitro-derived shoots to MS medium supplemented with 8.8 mM indole-3- butyric acid (IBA) and 1.43 mM indole-3-acetic acid resulted in root induction. In another treatment, IBA was found to be a good root-promoting agent, wherein dipping of explants in 4.4 μM IBA for 30 min and subsequent inoculation on MS basal medium was also beneficial for root induction. Successful field transfer was accomplished in rooted plantlets.
 
Date 2001
 
Type Article
PeerReviewed
 
Format application/pdf
 
Language en
 
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Identifier http://ir.cftri.com/2118/1/CURRENT_SCIENCE%2C_VOL._81%2C_NO._11%2C_10_DECEMBER_2001CURRENT_SCIENCE%2C_VOL._81%2C_NO._11%2C_10_DECEMBER_2001_1479.pdf
Obul Reddy, B. and Giridhar, P. and Ravishankar, G. A. (2001) In vitro rooting of Decalepis hamiltonii Wight & Arn., an endangered shrub, by auxins and root-promoting agents. Current Science, 81 (11). pp. 1479-1482.