Estimation of intracellular lipids by the measurement of absorbance of yeast cells stained with Sudan Black B
IR@CFTRI: CSIR-Central Food Technological Research Institute, Mysore
View Archive Info| Field | Value | |
| Relation |
http://ir.cftri.com/2038/
EMT-02-89 |
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| Title |
Estimation of intracellular lipids by the measurement of absorbance of yeast cells stained with Sudan Black B
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| Creator |
Thakur, M. S.
Prapulla, S. G. Karanth, N. G. |
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| Subject |
19 Yeast
01 Biotechnology and Bioengineering |
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| Description |
A rapid and sensitive method has been developed for the estimation of intracellular lipids accumulated in oleaginous yeast cells [Rhodotorula gracilis CFR 1 used for present studies]. The cells are stained with Sudan Black B and the absorbance is measured at 580 nm using unstained cells as control. A linear relationship is observed between absorbance and lipid concn. in fermentation broth. The values predicted using this relationship were compared with those obtained using the conventional method of solvent extraction followed by gravimetric estimation and a close agreement has been obtained.
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| Date |
1989
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| Type |
Article
PeerReviewed |
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| Format |
pdf
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| Language |
en
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| Identifier |
http://ir.cftri.com/2038/1/Enzyme%20Microb.%20Technol.%2C%201989%2C%20vol.%2011.pdf
Thakur, M. S. and Prapulla, S. G. and Karanth, N. G. (1989) Estimation of intracellular lipids by the measurement of absorbance of yeast cells stained with Sudan Black B. Enzyme and Microbial Technology, 11 (4). pp. 252-254. ISSN 0141-0229 |
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