A Spectrophotometric Method for Phosphine Residue Determination in Legumes
IR@CFTRI: CSIR-Central Food Technological Research Institute, Mysore
View Archive Info| Field | Value | |
| Relation |
http://ir.cftri.com/1584/
JFST-03-95 |
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| Title |
A Spectrophotometric Method for Phosphine Residue
Determination in Legumes
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| Creator |
Rangaswamy, J. R.
Gunasekaran, N. |
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| Subject |
22 Legumes-Pulses
05 Insect/Pest Control |
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| Description |
A simple spectrophotometric method for the determination of phosphine residue in legumes has been developed. It is based on the reaction of phosphine with silver nitrate in iso-propanol to form a pale yellow chromophore, with an absorption maximum at 436 nm. It gave a linear relationship between the absorbance at 436 nm and the amount of phosphine in the range of 0.02 to 0.48 µg/3 ml
reagent. The crop extractives from the legumes showed a low absorbance of 0.008 at 436 nm, and did not interfere in the chromophore reaction. The method is sensitive with determination limit of 0.015 µg phosphine and can be applied for determination of 0.02 µg residue in lOg legume samples. Phosphine residue on legumes, when extracted with a known volume of 2% aqueous isopropanolic silver nitrate, formed egg yellow chromophore with absorption maximum at the same range. Recovery of fortified phosphine from a closed system ranged from 97 to 98%.
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| Date |
1995
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| Type |
Article
PeerReviewed |
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| Format |
application/pdf
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| Language |
en
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| Rights |
—
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| Identifier |
http://ir.cftri.com/1584/1/JFST1995_32%281%29_42-45.pdf
Rangaswamy, J. R. and Gunasekaran, N. (1995) A Spectrophotometric Method for Phosphine Residue Determination in Legumes. Journal of Food Science and Technology, 32 (1). pp. 42-45. |
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