Quantification of aflatoxin and ochratoxin contamination in animal milk using UHPLC-MS/SRM method: a small-scale study.
IR@CFTRI: CSIR-Central Food Technological Research Institute, Mysore
View Archive Info| Field | Value | |
| Relation |
http://ir.cftri.com/14792/
https://doi.org/10.1007/s13197-021-04986-w |
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| Title |
Quantification of aflatoxin and ochratoxin contamination
in animal milk using UHPLC-MS/SRM method: a small-scale
study.
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| Creator |
Rukshan, Mehta
Sweekruthi, A. Shetty Melissa, F. Young Barry Ryan, P. Kannan, Rangiah |
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| Subject |
10 Food Microorganisms
27 Dairy products |
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| Description |
Mycotoxin contamination in animal milk is an
emerging concern around the globe. Here we developed
and validated an ultrahigh-performance liquid chromatography and mass spectrometry-selected reaction monitoring
(UHPLC/MS-SRM) method to quantify low concentrations
of aflatoxins (AFs) and ochratoxins (OTs) in routinely
consumed animal milk samples collected from southern
India. Stable isotope dilution methodology was applied to
quantify AFB1, AFB2, AFG1, AFG2, AFM1, AFM2 and
OTA, OTB in n = 38 different milk samples, using 1 mL
of milk. Bioanalytical parameters including method accuracy, precision, recovery, regression analysis and stability
were assessed. Dynamic ranges for quantification were
between 15.6–1000 pg/mL for AFB1, AFB2, AFG1, and
OTA; 7.8–500 pg/mL for AFM1, AFM2 and OTB;
78.6–5000 pg/mL for AFG2. Method accuracy ranged
between 80–120%, with ± 15% precision. Recoveries for
spiked standards were [ 88% in water and 75% in milk,
with limits of quantification (LOQ) ranging between
31.3 pg/mL for AFB1, AFB2, AFG1 and OTA, 15.6 pg/mL
for AFM1, AFM2 and OTB and 156 pg/mL for AFG2. R2
values for regression analyses ranged between
0.9991–0.9999. AFB2 [mean: 38 pg/mL (0.038 lg/kg)]
was quantified in goat milk, AFM1 was quantified in cow,
goat, pasteurized milk [mean: 331 pg/mL (0.331 lg/kg),
406 pg/mL (0.406 lg/kg), 164 pg/mL (0.164 lg/kg)].
Additionally, 90% of cow, goat and pasteurized milk
samples were above European Union (EU) limits of 50 pg/
mL (0.05 lg/kg) and 40% of cow and goat milk samples
were above the Food Safety Standards Authority of India
(FSSAI) limit of 500 pg/mL (0.5 lg/kg). AFM2 was also
quantified in cow, goat, and pasteurized milk samples
[mean: 249 pg/mL (0.249 lg/kg), 375 pg/mL (0.375 lg/
kg), 81 pg/mL (0.081 lg/kg)]. Our dynamic ranges for
quantification are lower than other published methods, with
need for a smaller volume of milk. This validated method
can be applied for routine quantification of mycotoxins in
milk.
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| Date |
2021
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| Type |
Article
PeerReviewed |
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| Format |
pdf
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| Language |
en
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| Identifier |
http://ir.cftri.com/14792/1/J%20Food%20Sci%20Technol%202021_1.pdf
Rukshan, Mehta and Sweekruthi, A. Shetty and Melissa, F. Young and Barry Ryan, P. and Kannan, Rangiah (2021) Quantification of aflatoxin and ochratoxin contamination in animal milk using UHPLC-MS/SRM method: a small-scale study. Journal of Food Science and Technology. ISSN 0022-1155 |
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