A bioactive polypeptide from sugarcane selectively inhibits intestinal sucrase.
IR@CFTRI: CSIR-Central Food Technological Research Institute, Mysore
View Archive Info| Field | Value | |
| Relation |
http://ir.cftri.com/14663/
https://doi.org/10.1016/j.ijbiomac.2020.03.085 |
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| Title |
A bioactive polypeptide from sugarcane selectively inhibits
intestinal sucrase.
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| Creator |
Shaik, Abduldileep
Narayanasamy, Raja Usharani, D. Ajeet, Singh Ram, Rajasekharan |
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| Subject |
05 Enzymes
25 Sugar/Starch/Confectionery |
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| Description |
Human sucrase enzyme is a key therapeutic target for type 2 diabetes. While sugarcane sucrase inhibitor (sucinh)
modulates invertase activity thereby accumulates sucrose. Molecular level understanding of sucinh towards
mammalian α-glucosidases is scarce. The interaction of sucinh with human sucrase was identified and the association of these proteins was confirmed using co-purification, co-immunoprecipitation and pull-down assay. In
addition, microscale thermophoresis assay showed that sucinh has a tight binding with sucrase (Kd =
4.77 nM) and a better affinity over acarbose. Collectively, in vitro, ex vivo and in silico data revealed that sucinh
is selective for intestinal sucrase. The M region (H5/6 loop) of sucinh identified at the protein-protein interface
is shown to have high affinity over N and C regions. Whereas, the biolayer luminescent imaging and microscale
thermophoresis on the synthetic peptide of 28 amino acids of M region has a weak dose-dependent binding with
sucrase. However, the synthetic peptide did not show substantial inhibition of sucrase and amylase activities at
low concentration. Naturally derived carbohydrate mimics were shown to have a positive impact at the in vitro
conditions. The insights obtained in this study give clues towards a new class of bioactive therapeutic peptides
for α-glucosidases. A new horizon towards polypeptides derived from food sources emerge as a promising strategy for dietary interventions for prediabetic conditions.
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| Date |
2020
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| Type |
Article
PeerReviewed |
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| Format |
pdf
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| Language |
en
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| Identifier |
http://ir.cftri.com/14663/1/International%20Journal%20of%20Biological%20Macromolecules%20156%20%282020%29%20938%E2%80%93948.pdf
Shaik, Abduldileep and Narayanasamy, Raja and Usharani, D. and Ajeet, Singh and Ram, Rajasekharan (2020) A bioactive polypeptide from sugarcane selectively inhibits intestinal sucrase. International Journal of Biological Macromolecules, 156. pp. 938-948. ISSN 0141-8130 |
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