Extracellular polysaccharide from Weissella confusa OF126: Production, optimization, and characterization.
IR@CFTRI: CSIR-Central Food Technological Research Institute, Mysore
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| Relation |
http://ir.cftri.com/13548/
https://doi.org/10.1016/j.ijbiomac.2018.01.060 |
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| Title |
Extracellular polysaccharide from Weissella confusa OF126: Production, optimization, and characterization.
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| Creator |
Adesulu Dahunsi, A. T.
Sanni, A. I. Jeyaram, K. Ojediran, J. O. Ogunsakin, A. O. |
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| Subject |
28 Polysaccharide Chemistry
02 Bacteriology |
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| Description |
The production, optimization, and characterization of exopolysaccharide (EPS) from
Weissella confusa OF126 and the in-vitro probiotic potentials of this strain was
investigated. The EPS produced on sucrose modified-MRS broth was characterized. The
purified EPS had an average molecular weight of 1.1x106 Da. HPLC analysis revealed the
presence of glucose monomers, indicating its homopolysaccharide nature. The structural
characteristics of the EPS were investigated by FTIR, and NMR spectroscopy. FTIR
spectroscopy revealed the presence of hydroxyl, carboxyl, N-acetyl and amine groups.
NMR analysis confirmed that the EPS contained α-(1→6) linkage and α-(1→3) branched
linkage. The EPS showed strong in-vitro antioxidant activity. Four significant factors were
optimized using Central Composite Design (CCD) and Response Surface Methodology
(RSM). The predicted optimum conditions for EPS production were cultivation time
(48.50 h), sucrose concentration (24.00 g/L), pH (7.00) and yeast extract (2.50 %).The
EPS produced was predicted to be 3.10 g/L, while the experimental yield was 3.00 g/L.
This strain was found to possess desirable probiotic attributes by its ability to survive at
pH 2.0 and in the presence of bile salts (0.50 % (w/v)) for 4 h. The results obtained from
this study demonstrate W. confusa OF126 as a promising probiotic and the EPS produced
can find useful applications in industries.
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| Date |
2018
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| Type |
Article
PeerReviewed |
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| Format |
pdf
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| Language |
en
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| Identifier |
http://ir.cftri.com/13548/1/Biomac%20%282018%29.pdf
Adesulu Dahunsi, A. T. and Sanni, A. I. and Jeyaram, K. and Ojediran, J. O. and Ogunsakin, A. O. (2018) Extracellular polysaccharide from Weissella confusa OF126: Production, optimization, and characterization. International Journal of Biological Macromolecules, 111. pp. 514-525. ISSN 0141-8130 |
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