High yield production of folates from soybean callus cultures in response to elicitors.
IR@CFTRI: CSIR-Central Food Technological Research Institute, Mysore
View Archive Info| Field | Value | |
| Relation |
http://ir.cftri.com/13538/
https://doi.org/10.1007/s13205-018-1101-x |
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| Title |
High yield production of folates from soybean callus cultures
in response to elicitors.
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| Creator |
Akitha Devi, M. K.
Sravan Kumar, Sandopu Giridhar, P. |
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| Subject |
01 Dietary Fiber
05 Soya bean |
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| Description |
Soybean (Indian variety, JS 335) callus growth and its folate content was sustained on Murashige and Skoog (MS) medium
supplemented with optimized levels of auxins and cytokinins. Callus cultures exhibited moderate production of total folate.
Callus growth was stimulated with highest biomass content of 21.3 g/L DW (24 days culture) on medium supplemented
with 1.5 mg/L 2,4-dichlorophenoxy acetic acid (2,4-D) and 0.1 mg/L Kinetin (Kn). Total folate production was enhanced by
an increase in indole-3-acetic acid (IAA) from 0.5 to 1.0 mg/L and decreased thereafter up to 1.5 mg/L. 2,4-D at 1.5 mg/L
repressed the callus growth and also folate production. A concentration of 1.0 mg/L IAA and 0.2 mg/L Kn elicited folate
production to a maximum total folate content of 323.82 μg/100 g DW. The results indicate that optimized medium for callus
suspension cultures conceivably are applied for scale-up studies in bioreactors.
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| Date |
2018
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| Type |
Article
PeerReviewed |
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| Format |
pdf
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| Language |
en
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| Identifier |
http://ir.cftri.com/13538/1/3%20Biotech%20%282018%29%20880.pdf
Akitha Devi, M. K. and Sravan Kumar, Sandopu and Giridhar, P. (2018) High yield production of folates from soybean callus cultures in response to elicitors. 3 Biotech, 8. pp. 1-10. |
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