A method for isolation of 2S, 7S, and 11S proteins of soyabean.
IR@CFTRI: CSIR-Central Food Technological Research Institute, Mysore
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http://ir.cftri.com/2476/
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| Title |
A method for isolation of 2S, 7S, and 11S proteins of soyabean.
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| Creator |
Appu Rao, A. G.
Narasinga Rao, M. S. |
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| Subject |
03 Proteins
05 Soya bean |
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| Description |
A method is described for the isolation of 2S, 7S and 11S proteins of soybean. The unfractionated proteins are first precipitated with Hg(II) which yields a 11S-rich precipitate and this is further purified by (NH4)2SO4precipitation. From Mg(II)supernatant which is rich in 7S and 2S proteins, they are separated by the use of (NH4)2SO4 and cold ethanol. The 7S and 11S protein are obtained in a highly purified form as indicated by ultracentrifugation and polyacrylamide gel electrophoresis.
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| Date |
1977
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| Type |
Article
PeerReviewed |
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| Format |
pdf
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| Language |
en
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| Identifier |
http://ir.cftri.com/2476/1/PREPARATIVE%20BIOCHEMISTRY%2C%207%282%29%2C%2089-101%20%281977%29.pdf
Appu Rao, A. G. and Narasinga Rao, M. S. (1977) A method for isolation of 2S, 7S, and 11S proteins of soyabean. Preparative Biochemistry, 7 (2). pp. 89-101. |
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