Development of media for growth of Dioscorea deltoidea cells in in vitro diosgenin production: Influence of media constituents and nutrient stress.
IR@CFTRI: CSIR-Central Food Technological Research Institute, Mysore
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http://ir.cftri.com/3769/
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| Title |
Development of media for growth of Dioscorea deltoidea cells in in vitro diosgenin production: Influence of media constituents and nutrient stress.
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| Creator |
Ravishankar, G. A.
Grewal, C. |
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| Subject |
10 Plants
05 Tissue Culture |
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| Description |
Callus culture of Dioscorea deltoidea produced diosgenin and sterols during
stationary phase. Ammonium nitrate (420 mg Nitrogen/l) as sole nitrogen source
supported better growth than a combination of ammonium nitrate and potassium
nitrate (totally equivalent to 840 mg Nitrogen/l). The production of diosgenin
increased under low phosphate concentration (100 mg/l) whereas high phosphate
concentration (240 mg/!) promoted growth. Micronutrients, when used at 1ı89
strength, enhanced growth and diosgenin production. Depletion of nitrogen
increase in the diosgenin synthesis by a factor of 2. Adoption of a two stage
culture method enhanced the diosgenin production in cultured cells by eight-fold.
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| Date |
1991
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| Type |
Article
PeerReviewed |
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| Format |
pdf
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| Language |
en
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| Identifier |
http://ir.cftri.com/3769/1/Biotechnology%20Letters%20Vol%2013%20No%202%20125-130%20%281991%29.pdf
Ravishankar, G. A. and Grewal, C. (1991) Development of media for growth of Dioscorea deltoidea cells in in vitro diosgenin production: Influence of media constituents and nutrient stress. Biotechnology Letters, 13 (2). pp. 125-130. |
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