Effect of sodium dodecyl sulfate, acid, alkali, urea and guanidine hydrochloride on the circular dichroism of alpha-globulin of Sesamum indicum L.
IR@CFTRI: CSIR-Central Food Technological Research Institute, Mysore
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http://ir.cftri.com/4006/
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| Title |
Effect of sodium dodecyl sulfate, acid, alkali, urea and guanidine hydrochloride on the circular dichroism of alpha-globulin of Sesamum indicum L.
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| Creator |
Prakash, V.
Nandi, P. K. Jirgensons, B. |
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| Subject |
03 Proteins
01 Oilseeds |
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| Description |
The circular dichroic spectra of a-globulin have been obtained under various
solution conditions of sodium dodecyl sulfate, acid, alkali, urea and guanidine
hydrochloride. The protein in phosphate buffer pH 7.4, 0 . 2 , h~a s about 25% bstructure
and 5% a-helix, the rest being aperiodic or irregular structure. Sodium
dodecyl sulfate induced more a-helical structure in the protein. The protein had
nearly 20% a-helix at 1 x lo-= M SDS. At extreme acid or alkaline pH, the
protein had no a-helix with Pstructure decreasing with further extremes of pH.
The protein is represented by 100% aperiodic structure in 6.6 m urea and in 6.0 m guanidine hydrochloride solutions. The above results are discussed in view
of some of the earlier results with regard to the association-dissociation and
denaturation behavior of a-globulin under various solution conditions.
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| Date |
1980
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| Type |
Article
PeerReviewed |
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| Format |
pdf
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| Language |
en
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| Identifier |
http://ir.cftri.com/4006/1/Int.%20J.%20Peptide%20Protein%20Res.%2015%2C1980%2C305-313.pdf
Prakash, V. and Nandi, P. K. and Jirgensons, B. (1980) Effect of sodium dodecyl sulfate, acid, alkali, urea and guanidine hydrochloride on the circular dichroism of alpha-globulin of Sesamum indicum L. International Journal of Peptide and Protein Research, 15. pp. 305-313. |
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