Enzymatic synthesis of cholecalciferol glycosides using β-glucosidase from sweet almond
IR@CFTRI: CSIR-Central Food Technological Research Institute, Mysore
View Archive Info| Field | Value | |
| Relation |
http://ir.cftri.com/9820/
JFST-148-10 |
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| Title |
Enzymatic synthesis of cholecalciferol glycosides
using β-glucosidase from sweet almond
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| Creator |
Manohar, Balaraman
Divakar, S. |
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| Subject |
33 Nuts
34 Vitamin Chemistry |
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| Description |
β-Glucosidase from sweet almond (Amygdalus
communis var. ‘Dulcis’) entrapped on to calcium alginate
beads catalysed the synthesis of water soluble 17-O-(Dglucopyranosyl)
cholecalciferol. Optimum conditions for the
reaction were: 60% (w/w D-glucose) β-glucosidase,
0.12 mM pH 6 phosphate buffer and 30 h incubation period.
β-Glucosidase also catalyzed the reaction with D-glucose 2,
D-galactose 3, D-mannose 4 and D-fructose 5 with generally
low yields in the range of 3–14%. Both α/β anomers of Dglucose
2, D-galactose 3 and D-mannose 4 reacted, of which
the former two formed C6-O derivatives also.
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| Date |
2010
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| Type |
Article
PeerReviewed |
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| Format |
application/pdf
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| Language |
en
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| Identifier |
http://ir.cftri.com/9820/1/J_Food_Sci_Technol__2010_4.pdf
Manohar, Balaraman and Divakar, S. (2010) Enzymatic synthesis of cholecalciferol glycosides using β-glucosidase from sweet almond. Journal of Food Science and Technology, 47 (5). pp. 469-475. |
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