Diet-induced hypercholesterolemia alters liver glycosaminoglycans and associated-lipoprotein receptors in rats.
IR@CFTRI: CSIR-Central Food Technological Research Institute, Mysore
View Archive Info| Field | Value | |
| Relation |
http://ir.cftri.com/13139/
http://dx.doi.org/10.1007/s13105-017-0583-z |
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| Title |
Diet-induced hypercholesterolemia alters liver
glycosaminoglycans and associated-lipoprotein receptors in rats.
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| Creator |
Kiran, G.
Prasada Rao, U. J. S. Salimath, P. V. Nandini, C. D. |
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| Subject |
28 Polysaccharide Chemistry
04 Diabetes Mellitus |
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| Description |
Glycosaminoglycans (GAGs) play an important
role in lipoprotein metabolism. In liver, it facilitates the
uptake of remnants through receptor-independent endocytosis.
However, changes in liver GAGs during diet-induced
hypercholesterolemia with normal levels of fat feeding are
unknown. Present paper highlights the effect of dietinduced
hypercholesterolemia with normal levels (5%) of
fat on liver GAGs and other associated lipoprotein receptors.
Hypercholesterolemia was induced in rats by feeding
diet supplemented with 0.5% cholesterol and 0.125% bile
salts. Hypercholesterolemia showed significantly decreased
GAGs of both heparan sulfate (HS) and chondroitin
sulfate/dermatan sulfate (CS/DS) classes of molecules.
Quantitative real-time polymerase chain reaction analysis
of GAG biosynthetic enzymes and other genes revealed
significant changes in expression profile. The decrease in
GAGs was prevented by simvastatin treatment; a drug that
inhibits endogenous cholesterol synthesis that was used as
a positive control in our study. Furthermore, there was a
comparatively decreased binding of GAGs from hypercholesterolemic
rats to lipoprotein lipase. LRP1 which plays a
major role in lipoprotein uptake was also significantly decreased,
and it was attenuated in simvastatin-treated hypercholesterolemic
rats. Furthermore, LDLR and ApoE were
also decreased significantly in liver of hypercholesterolemic
rats. Thus, diet-induced hypercholesterolemia results in dysregulation
of cholesterol homeostasis apparently through
changes in GAGs in conjunction with other associated
players.
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| Date |
2017
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| Type |
Article
PeerReviewed |
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| Format |
pdf
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| Language |
en
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| Identifier |
http://ir.cftri.com/13139/1/J%20Physiol%20Biochem%20%282017%29%2073539%E2%80%93550.pdf
Kiran, G. and Prasada Rao, U. J. S. and Salimath, P. V. and Nandini, C. D. (2017) Diet-induced hypercholesterolemia alters liver glycosaminoglycans and associated-lipoprotein receptors in rats. Journal of Physiology and Biochemistry, 73. pp. 539-550. |
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