A soluble diacylglycerol acyltransferase is involved in triacylglycerol biosynthesis in the oleaginous yeast Rhodotorula glutinis.
IR@CFTRI: CSIR-Central Food Technological Research Institute, Mysore
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| Title |
A soluble diacylglycerol acyltransferase is involved
in triacylglycerol biosynthesis in the oleaginous
yeast Rhodotorula glutinis.
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| Creator |
Sapa, Hima Rani
Saha, Saikat Ram, Rajasekharan |
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| Subject |
16 Enzyme Chemistry
19 Yeast |
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| Description |
The biosynthesis of triacylglycerol (TAG) occurs in the microsomal membranes of eukaryotes.
Here, we report the identification and functional characterization of diacylglycerol acyltransferase
(DGAT), a member of the 10 S cytosolic TAG biosynthetic complex (TBC) in Rhodotorula
glutinis. Both a full-length and an N-terminally truncated cDNA clone of a single gene were
isolated from R. glutinis. The DGAT activity of the protein encoded by RgDGAT was confirmed in
vivo by the heterologous expression of cDNA in a Saccharomyces cerevisiae quadruple mutant
(H1246) that is defective in TAG synthesis. RgDGAT overexpression in yeast was found to be
capable of acylating diacylglycerol (DAG) in an acyl-CoA-dependent manner. Quadruple mutant
yeast cells exhibit growth defects in the presence of oleic acid, but wild-type yeast cells do not. In
an in vivo fatty acid supplementation experiment, RgDGAT expression rescued quadruple mutant
growth in an oleate-containing medium. We describe a soluble acyl-CoA-dependent DAG
acyltransferase from R. glutinis that belongs to the DGAT3 class of enzymes. The study highlights
the importance of an alternative TAG biosynthetic pathway in oleaginous yeasts.
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| Date |
2013
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| Type |
Article
PeerReviewed |
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| Format |
application/pdf
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| Identifier |
http://ir.cftri.com/11071/1/Micrbiol%20Reprint.pdf
Sapa, Hima Rani and Saha, Saikat and Ram, Rajasekharan (2013) A soluble diacylglycerol acyltransferase is involved in triacylglycerol biosynthesis in the oleaginous yeast Rhodotorula glutinis. Microbiology, 159. pp. 155-166. |
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| Relation |
http://ir.cftri.com/11071/
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