DEVELOPMENT AND OPTIMIZATION OF AN HPLC METHOD FOR THE ROUTINE ANALYSIS OF CATECHINS, CAFFEINE, AND GALLIC ACID IN TEA (CAMELLIA SINENSIS)
IR@IHBT: CSIR-Institute of Himalayan Bioresource Technology, Palampur
View Archive Info| Field | Value | |
| Title |
DEVELOPMENT AND OPTIMIZATION OF AN HPLC METHOD FOR
THE ROUTINE ANALYSIS OF CATECHINS, CAFFEINE, AND GALLIC
ACID IN TEA (CAMELLIA SINENSIS)
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| Creator |
Sharma, Anupma
Bhondekar, Amol P Bari, S S Gulati, Ashu Kapur, Pawan Singla, M L |
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| Subject |
Plant viruses
Microbiology |
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| Description |
An efficient and rapid high performance liquid chromatographic (HPLC) assay was developed
for the quantification of catechins [(þ) – catechin, (�) – epigallocatechin, (�) – epigallocatechingallate,
(�) – epicatechin, and (�) – epicatechingallate], caffeine, and gallic acid in tea (Camellia
Sinesis var. sinesis). The assay was optimized by varying sample strength, column temperature,
gradient type, and detection wavelength. A curved gradient using a Thermo Hypersil ODS column
with 0.05% orthophosphoric acid and methanol as mobile phase A and B, respectively, and UV
detection at 277.5 nm was employed. It was observed that a curved gradient along with an optimal
temperature, dramatically improves the signal to noise ratio and separation profile. The limit
of detection (LOD) and limit of quantification (LOQ) were found to be in the range of
1.04–22.81 lgmL�1 and 3.47–76.05 lgmL�1 respectively. The overall precision values obtained
from the analysis of Kangra and Darjeeling orthodox tea samples were within the range of 0–0.34
(standard error).
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| Date |
2011
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| Type |
Article
PeerReviewed |
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| Format |
application/pdf
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| Identifier |
http://ihbt.csircentral.net/1062/1/98_Development_2011.pdf
Sharma, Anupma and Bhondekar, Amol P and Bari, S S and Gulati, Ashu and Kapur, Pawan and Singla, M L (2011) DEVELOPMENT AND OPTIMIZATION OF AN HPLC METHOD FOR THE ROUTINE ANALYSIS OF CATECHINS, CAFFEINE, AND GALLIC ACID IN TEA (CAMELLIA SINENSIS). Journal of Liquid Chromatography & Related Technologies,, 34. pp. 2243-2255. |
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| Relation |
http://ihbt.csircentral.net/1062/
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