Identification and expression analysis of CjLTI, a novel low temperature responsive gene from Caragana jubata
IR@IHBT: CSIR-Institute of Himalayan Bioresource Technology, Palampur
View Archive Info| Field | Value | |
| Title |
Identification and expression analysis of CjLTI, a novel low
temperature responsive gene from Caragana jubata
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| Creator |
Bhardwaj, P K
Kaur, J Sobti, R C Kumar, Sanjay |
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| Subject |
Plant sciences
Natural Product Chemistry |
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| Description |
Abstract Using rapid amplification of cDNA ends, a full
length cDNA (CjLTI) was cloned from apical buds of
Caragana jubata, a plant species that grows under extreme
cold. The cDNA obtained was 573 bp long consisting of an
open reading frame of 351 bp encoding 116 amino acids.
Homology analysis did not exhibit significant similarity
with any sequence at NCBI database, therefore it was
deduced as a novel gene. Secondary structure analysis
suggested that the deduced CjLTI contained 25.86% ahelices,
4.31% b-turns, 6.90% extended strands, and
62.93% random coils. The hydropathy profile suggested
CjLTI to be a hydrophobic protein having characteristic
features of signal peptides at N-terminus. The gene
exhibited down-regulation at 5 min of exposure to low
temperature (LT, 4 ± 3�C) followed by a strong up-regulation
after 15 min and onwards. Methyl jasmonate (MJ)
lead to up-regulation of CjLTI starting at 5 min onwards.
The gene exhibited up- and down-regulation of expression
pattern in response to abscisic acid (ABA) and salicylic
acid (SA). Mild drought stress slightly up-regulated gene
expression and at severe drought (up to 115% reduction in
leaf water potential) slight down-regulation of gene
expression was observed. These results suggested CjLTI to
be a LT responsive gene wherein MJ, ABA and SA pathways
might be involved in regulating the gene expression.
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| Date |
2012
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| Type |
Article
PeerReviewed |
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| Format |
application/pdf
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| Identifier |
http://ihbt.csircentral.net/958/1/09%2D02%2D2012_Identification.pdf
Bhardwaj, P K and Kaur, J and Sobti, R C and Kumar, Sanjay (2012) Identification and expression analysis of CjLTI, a novel low temperature responsive gene from Caragana jubata. Journal of Molecular Biology Reports, 39. pp. 3197-3202. |
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| Relation |
http://ihbt.csircentral.net/958/
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