Factors affecting in vitro formation of cormlets in Gladiolus hybridus Hort. and their field performance
IR@IHBT: CSIR-Institute of Himalayan Bioresource Technology, Palampur
View Archive Info| Field | Value | |
| Title |
Factors affecting in vitro formation of cormlets
in Gladiolus hybridus Hort. and their field performance
|
|
| Creator |
Kumar, Anil
Palni, LMS Sood, A |
|
| Subject |
Plant Biotechnology
Plant viruses |
|
| Description |
Abstract The effect of number of important factors on in
vitro cormlet formation has been investigated in Gladiolus
hybridus Hort. Sucrose concentration of 232 mM was
found to be best for producing higher number of cormlets
per flask, whereas, the average mass of a cormlet increased
with increase in sucrose concentration. Amongst three
cultivars (cvs), maximum number of cormlets produced per
flask was recorded in cv ‘Her Majesty’, but the average
cormlet mass was higher in case of cv ‘Bright Eye’.
Although the number of cormlets produced was found to be
marginally higher at 30�C when compared with 20�C, the
average cormlet mass was higher at the lower temperature.
Both the number of cormlets formed per culture flask as
well as the average fresh mass of a cormlet increased with
increase in the size (volume) of the culture flask used. The
known inhibitors of gibberellin biosynthesis used in this
study suppressed cormlet formation, and the maximum
inhibition was recorded in case of maleic acid hydrazide.
Polyamines were found to be beneficial for cormlet
formation, and amongst the polyamines used, incorporation
of spermidine in the culture medium resulted in maximum
number of cormlet formation per culture flask. Field trials
indicated that the performance of such in vitro produced
cormlets was comparable to that of conventionally produced
cormels of the same weight range. The plants raised
from in vitro produced cormlets were found to be morphologically
similar to the mother plant.
|
|
| Date |
2011
|
|
| Type |
Article
PeerReviewed |
|
| Format |
application/pdf
|
|
| Identifier |
http://ihbt.csircentral.net/951/1/08%2D02%2D2012_Factors.pdf
Kumar, Anil and Palni, LMS and Sood, A (2011) Factors affecting in vitro formation of cormlets in Gladiolus hybridus Hort. and their field performance. Acta. Physiol Plant, 33. pp. 509-515. |
|
| Relation |
http://ihbt.csircentral.net/951/
|
|