Amelioration of oxidative damage by Methyl gallate in different in vitro models
IR@IHBT: CSIR-Institute of Himalayan Bioresource Technology, Palampur
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| Title |
Amelioration of oxidative damage by Methyl gallate in different in
vitro models
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| Creator |
Kaur, R
Singh , Bikram Arora, S |
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| Subject |
Plant sciences
Natural Product Chemistry |
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| Description |
Abstract
The present study aims to evaluate the antioxidant potential of methyl gallate - an
ester of gallic acid in different in vitro assays. The antioxidant capacity was
assessed using Molybdate ion reduction, DPPH, ABTS radical cation scavenging,
deoxyribose degradation (site specific and non-site specific), reducing power, lipid
peroxidation, superoxide anion scavenging, chelating power and DNA nicking
assays and the results were compared with known antioxidant i.e. gallic acid. From
the results obtained, it was found that methyl gallate exhibited potent antioxidant
activities in different in vitro models and the activity was related to its chemical
structure. The reduction ability of methyl gallate was found to be 99.58mg AAE
/100mg dry weight of pure compound. The IC50 value of methyl gallate in DPPH,
ABTS.+ scavenging, deoxyribose degradation (site specific and non-site specific),
reducing power, lipid peroxidation, superoxide anion scavenging, chelating power
was found to be 21.679, 8.689, 19.884 and 20.086, 91.169, 15.825, 32.699 and
4990.2 μg/ml respectively. The methyl gallate also has tendency to conserve the
supercoiled DNA (pBR 322) from the .OH radicals mediated damage in DNA
nicking assay. However, it has been found that hydrogen donation, free radical
scavenging potential of methyl gallate was lower than standard antioxidant
compound i.e. gallic acid and the lower effect was related to substitution of –OH
group by –CH3 group.
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| Date |
2011
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| Type |
Article
PeerReviewed |
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| Format |
application/pdf
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| Identifier |
http://ihbt.csircentral.net/945/1/08%2D02%2D2012_Amelioration.pdf
Kaur, R and Singh , Bikram and Arora, S (2011) Amelioration of oxidative damage by Methyl gallate in different in vitro models. Phytopharmacology, 1 (4). pp. 82-94. |
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| Relation |
http://ihbt.csircentral.net/945/
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