In vivo protein trapping produces a functional expression codex of the vertebrate proteome
IR@IGIB: CSIR-Institute of Genomics & Integrative Biology, New Delhi
View Archive Info| Field | Value | |
| Title |
In vivo protein trapping produces a functional
expression codex of the vertebrate proteome
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| Creator |
Clark, Karl J
Balciunas, Darius Pogoda, Hans-Martin Ding, Yonghe Westcot, Stephanie E Bedell, Victoria M Greenwood, Tammy M Urban, Mark D Skuster, Kimberly J Petzold, Andrew M Ni, Jun Nielsen, Aubrey L Patowary , Ashok Scaria, Vinod Sivasubbu, Sridhar Xu, Xiaolei Hammerschmidt, Matthias Ekker, Stephen C |
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| Subject |
G1 Genome informatics (General)
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| Description |
We describe a conditional in vivo protein-trap mutagenesis system that reveals spatiotemporal protein expression dynamics and can be used to assess gene function in the vertebrate Danio rerio. Integration of pGBT-RP2.1 (RP2), a gene-breaking transposon containing a protein trap, efficiently disrupts gene expression with >97% knockdown of normal transcript amounts and simultaneously reports protein expression for each locus. The mutant alleles are revertible in somatic tissues via Cre recombinase or splice-site-blocking morpholinos and are thus to our knowledge the first systematic conditional mutant alleles outside the mouse model. We report a collection of 350 zebrafish lines that include diverse molecular loci. RP2 integrations reveal the complexity of genomic architecture and gene function in a living organism and can provide information on protein subcellular localization. The RP2 mutagenesis system is a step toward a unified 'codex' of protein expression and direct functional annotation of the vertebrate genome.
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| Date |
2011-05-08
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| Type |
Article
PeerReviewed |
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| Format |
application/pdf
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| Identifier |
http://openaccess.igib.res.in/138/1/nmeth.1606.pdf
Clark, Karl J and Balciunas, Darius and Pogoda, Hans-Martin and Ding, Yonghe and Westcot, Stephanie E and Bedell, Victoria M and Greenwood, Tammy M and Urban, Mark D and Skuster, Kimberly J and Petzold, Andrew M and Ni, Jun and Nielsen, Aubrey L and Patowary , Ashok and Scaria, Vinod and Sivasubbu, Sridhar and Xu, Xiaolei and Hammerschmidt, Matthias and Ekker, Stephen C (2011) In vivo protein trapping produces a functional expression codex of the vertebrate proteome. In vivo protein trapping produces a functional expression codex of the vertebrate proteome, 8 (6). pp. 506-515. |
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| Relation |
http://openaccess.igib.res.in/138/
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