Cryopreservation studies on the marine diatom Navicula subinflata Grun
IR@NIO: CSIR-National Institute Of Oceanography, Goa
View Archive Info| Field | Value | |
| Creator |
Redekar, P.D.
Wagh, A.B. |
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| Date |
2006-10-17T11:55:59Z
2006-10-17T11:55:59Z 2000 |
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| Identifier |
Seaweed Res. Util., vol.22(1&2), 183-187p.
http://drs.nio.org/drs/handle/2264/442 |
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| Description |
Very little work has been done on marine unicellular algae regarding cryopreservation. The present work was, therefore, undertaken to study the effect of different cryoprotectants and cryopreservation on the growth of marine diatom Navicula subinflata. The effect of cryoprotectants such as Ethylene glycol, DMSO, Methanol, Glycerol and Sucrose was studied on Navicula subinflata at 1M, 2M, 4M and 6M concentrations for a period of 30 and 60 minutes to find out the optimum concentration for the growth of diatom for cryopreservation experiment. The optimum concentration of Ethylene glycol, DMSO and Methanol was found to be 4M in 30 minute experiment, while in 60 minute experiment no satisfactory growth was observed and increasing concentration of cryoprotectant decreased the growth. These experiments after cryopreservation revealed that the maximum growth was recorded in Ethylene glycol treated cells for 0 day sample and 7 days and 30 days liquid nitrogen preserved samples. No growth was observed in Methanol (4M) treated cells for 7 days, 15 and 30 days liquid nitrogen preserved samples. Thus the present work reveals the possibility of application of cryopreservation in marine algae.
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| Format |
69142 bytes
application/pdf |
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| Language |
en
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| Publisher |
Seaweed research and Utilization Association
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| Rights |
This full-text version of the paper hosted with the permission
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| Subject |
marine unicellular algae
marine diatom |
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| Title |
Cryopreservation studies on the marine diatom Navicula subinflata Grun
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| Type |
Article
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