Phylogenetic Characterization of Archaea in Saltpan Sediments
IR@IIIM: CSIR-Indian Institute of Integrative Medicine, Jammu
View Archive Info| Field | Value | |
| Title |
Phylogenetic Characterization of Archaea in Saltpan Sediments
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| Creator |
Ahmad, Nasier
Johri, Sarojini Sultan, Phalisteen Abdin, Malik Z. Qazi, Ghulam N. |
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| Subject |
Biological Sciences
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| Description |
A study was undertaken to investigate the presence of archaeal diversity in saltpan sediments of Goa,
India by 16S rDNA-dependent molecular phylogeny. Small
subunit rRNA (16S rDNA) from saltpan sediment metagenome were amplified by polymerase chain reaction(PCR) using primers specific to the domain archaea. 10 unique phylotypes were obtained by PCR based RFLP of 16S rRNA genes using endonuclease Msp 1, which was most suitable to score the genetic diversity. These phylotypes spanned a wide range within the domain archaea including both crenarchaeota and euryarcheaota. None of the retrieved crenarchaeota sequences could be grouped with previously cultured crenarchaeota however; two sequences were related with haloarchaea. Most of the sequences determined were closely related to the sequences that had been previously obtained from metagenome of a variety of marine environments. The phylogenetic study of a site investigated for the first time revealed the presence of low archaeal population but showed yet unclassified species, may specially adapted to the salt pan sediment of Goa.
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| Date |
2011
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| Type |
Article
PeerReviewed |
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| Format |
application/pdf
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| Identifier |
http://iiim.csircentral.net/307/1/10.1007s12088%2D011%2D0125%2D2.pdf
Ahmad, Nasier and Johri, Sarojini and Sultan, Phalisteen and Abdin, Malik Z. and Qazi, Ghulam N. (2011) Phylogenetic Characterization of Archaea in Saltpan Sediments. Indian Journal of Microbiology, 51 (2). pp. 132-137. ISSN 0046-8991 |
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| Relation |
http://dx.doi.org/10.1007/s12088-011-0125-2
http://iiim.csircentral.net/307/ |
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