Isolation and purification of acetylshikonin and b-acetoxyisovalerylshikonin from cell suspension cultures of Arnebia euchroma (Royle) Johnston using rapid preparative HPLC
IR@IHBT: CSIR-Institute of Himalayan Bioresource Technology, Palampur
View Archive Info| Field | Value | |
| Title |
Isolation and purification of acetylshikonin and
b-acetoxyisovalerylshikonin from cell suspension
cultures of Arnebia euchroma (Royle) Johnston
using rapid preparative HPLC
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| Creator |
Sharma , N
Sharma, U K Malik, S Bhushan, Shashi Kumar, Vinod Verma , S C Sharma, N Sharma, Madhu Sinha, Arun K |
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| Subject |
Natural Product Chemistry
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| Description |
Shikonin and its derivatives are important red colored naphthoquinone pigments
found in a large number of Arnebia species, including A. euchroma, that are responsible
for the various pharmacological activities exhibited by the plant. The precise
separation of each naphthoquinone is essential for total quality evaluation and bioactivity
analysis of herbal formulations of A. euchroma. Furthermore, the overexploitation
of this useful plant has resulted in species becoming endangered. With this
in mind, a simple and rapid preparative scale HPLC method with single compound
recovery for the isolation and purification of two shikonin derivatives (i. e. acetylshikonin,
b-acetoxyisovalerylshikonin) from cell suspension cultures of A. euchroma is
presented. The compounds were separated on a C18 column within 10 min using acetonitrile/
methanol (95:5) as mobile phase in isocratic mode. The isolated compounds
were found to be more than 98% pure. The LOD for acetylshikonin and bacetoxyisovalerylshikonin
was estimated at 0.063 and 0.146 lg/mL, respectively,
while the LOQ was found to be 0.209 and 0.487 lg/mL, respectively. The recoveries
accomplished for both the shikonin derivatives were in the range of 94.7–96.8%.
The repeatability, expressed as %RSD, of acetylshikonin and b-acetoxyisovalerylshikonin
was found to be 1.74 and 1.27, respectively.
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| Date |
2008
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| Type |
Article
PeerReviewed |
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| Format |
application/pdf
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| Identifier |
http://ihbt.csircentral.net/527/1/383_2008_2%2D12%2D2011.pdf
Sharma , N and Sharma, U K and Malik, S and Bhushan, Shashi and Kumar, Vinod and Verma , S C and Sharma, N and Sharma, Madhu and Sinha, Arun K (2008) Isolation and purification of acetylshikonin and b-acetoxyisovalerylshikonin from cell suspension cultures of Arnebia euchroma (Royle) Johnston using rapid preparative HPLC. Journal of Separation Science, 31. pp. 629-635. |
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| Relation |
http://ihbt.csircentral.net/527/
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