Online HPLC-DPPH method for antioxidant activity of Picrorhiza kurroa Royle ex Benth. and characterization of kutkoside by Ultra-Performance LC-electrospray ionization quadrupole time-of-flight mass spectrometry
IR@IHBT: CSIR-Institute of Himalayan Bioresource Technology, Palampur
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| Title |
Online HPLC-DPPH method for antioxidant activity of Picrorhiza kurroa Royle
ex Benth. and characterization of kutkoside by Ultra-Performance LC-electrospray
ionization quadrupole time-of-flight mass spectrometry
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| Creator |
Bhandari, P
Singh , Bikram Kumar, Neeraj |
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| Subject |
Natural Product Chemistry
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| Description |
Picrorhiza kurroa Royle ex Benth., is widely used in the Indian systems of medicine for the treatment of various liver ailments. Since,
the role of oxidative stress in the pathogenesis of liver injury has become generally recognized, in present study the free radical
scavenging effect of P. kurroa was assessed by on-line HPLC-DPPH and colorimetric DPPH methods. The comparative study on
antioxidant activity of P. kurroa extracts by both methods revealed that colorimetric method showed very less free radical scavenging
effect while HPLC-DPPH method showed high activity. Further, the kutkoside, an important ingredient of a potent hepatoprotective
formulation “kutkin/ picroliv” was investigated for its chemical composition by ultra-performance liquid chromatography coupled with
diode array detection/electrospray ionization quadrupole time-of-flight mass spectrometry (UPLC-DAD/ESI-QTOF-MS). Kutkoside was
considered to be a single compound and reported as picroside-II or kutkoside, however, present investigation illustrated that kutkoside is
a mixture of iridoid glycosides namely, picroside II, picroside IV and 6-ferulloylcatalpol.
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| Date |
2010
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| Type |
Article
PeerReviewed |
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| Format |
application/pdf
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| Identifier |
http://ihbt.csircentral.net/201/1/Exp_Botany.pdf
Bhandari, P and Singh , Bikram and Kumar, Neeraj (2010) Online HPLC-DPPH method for antioxidant activity of Picrorhiza kurroa Royle ex Benth. and characterization of kutkoside by Ultra-Performance LC-electrospray ionization quadrupole time-of-flight mass spectrometry. Indian Journal of Experimental Biology, 48. pp. 323-328. |
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| Relation |
http://ihbt.csircentral.net/201/
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