Detection, identification and incidence of Chrysanthemum B carlavirus in chrysanthemum in India
IR@IHBT: CSIR-Institute of Himalayan Bioresource Technology, Palampur
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| Title |
Detection, identification and incidence of Chrysanthemum B
carlavirus in chrysanthemum in India
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| Creator |
Verma, N
Sharma, A Hallan, Vipin Raja, Ram |
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| Subject |
Plant viruses
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| Description |
During a survey of chrysanthemum cvs. grown in Himachal Pradesh. (India) a variety of symptoms were seen. The disease
incidence ranged between 40.62% and 94.66%. Out of 36 cvs. surveyed 7 were found to be negative for CBV in DAS-ELISA. The
virus has a very narrow host range infecting only N. clevelandii, N. glutinosa, N. rustica, Petunia hybrida and Vicia faba. In cytopathology infected cells showed absence of specific inclusion body (ies), presence of virus particles in large numbers along the
outer envelope of chloroplast with abnormalities like electron dense matrix, loss of chloroplast envelope and extensive invegination
of cytoplasm. In immune electron microscopy there was heavy decoration and trapping of virus particle with the antiserum specific
to CBV. The particle length was ca. 680�12 nm. The causal virus was found to be Chrysanthemum B carlavirus on the basis of host
range, DAS-ELISA, immune electron microscopy and cytopathology. This may have practical benefits for producing virus-free
stocks of chrysanthemum for growers
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| Date |
2003
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| Type |
Article
PeerReviewed |
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| Format |
application/pdf
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| Identifier |
http://ihbt.csircentral.net/197/1/CVB_Detection.pdf
Verma, N and Sharma, A and Hallan, Vipin and Raja, Ram (2003) Detection, identification and incidence of Chrysanthemum B carlavirus in chrysanthemum in India. Crop Protection, 22. pp. 425-429. |
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| Relation |
http://ihbt.csircentral.net/197/
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