Agrobacterium-mediated genetic transformation of tea leaf explants: effects of counteracting bactericidity of leaf polyphenols without loss of bacterial virulence
IR@IHBT: CSIR-Institute of Himalayan Bioresource Technology, Palampur
View Archive Info| Field | Value | |
| Title |
Agrobacterium-mediated genetic transformation of tea leaf
explants: effects of counteracting bactericidity of leaf polyphenols
without loss of bacterial virulence
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| Creator |
Sandal, I
Saini, U Bhattacharya, Amita Ahuja, Paramvir Singh Lacroix, B Citovsky, V |
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| Subject |
Plant sciences
Microbiology |
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| Description |
Tea is one of the major crops in Asia and Africa,
and its improvement by genetic modification is important for
economy of many tea-producing regions. Although somatic
embryos derived from cotyledon explants have been transformed
with Agrobacterium, the leaves of several commercially
important tea cultivars have remained recalcitrant to
transformation, largely due to bactericidal effect of polyphenols
that are exuded by tea leaves in vitro.Moreover, the commonly
used polyphenol adsorbents and antioxidants cannot
overcome this problem. Leaf explants, however, are more desirable
than cotyledon-derived somatic embryos, especially
when it is necessary to further improve a selected elite and
also retain its superior traits. Thus, we developed a procedure
for Agrobacterium-mediated genetic transformation
of tea leaf explants which is based on the presence of lglutamine
in the co-cultivation medium. We then showed
that the transformation process is facilitated via a protective
action of l-glutamine against bactericidal effects of leaf
polyphenols without affecting the bacterial virulence (vir)
gene expression.
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| Date |
2007
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| Type |
Article
PeerReviewed |
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| Format |
application/pdf
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| Identifier |
http://ihbt.csircentral.net/118/1/2006_Agrobacterium.pdf
Sandal, I and Saini, U and Bhattacharya, Amita and Ahuja, Paramvir Singh and Lacroix, B and Citovsky, V (2007) Agrobacterium-mediated genetic transformation of tea leaf explants: effects of counteracting bactericidity of leaf polyphenols without loss of bacterial virulence. Plant Cell Reports, 26 (2). pp. 169-176. |
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| Relation |
http://ihbt.csircentral.net/118/
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