Transient RNAi based gene silencing of glutathione synthetase reduces glutathione content in Camellia sinensis (L.) O. Kuntze somatic embryos
IR@IHBT: CSIR-Institute of Himalayan Bioresource Technology, Palampur
View Archive Info| Field | Value | |
| Title |
Transient RNAi based gene silencing of glutathione synthetase reduces
glutathione content in Camellia sinensis (L.) O. Kuntze somatic embryos
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| Creator |
Mohanpuria, P
Rana, N K Yadav , S K |
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| Subject |
Plant Biotechnology
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| Description |
We report on gene silencing of glutathione synthetase (GSHS) that reduces reduced glutathione (GSH) content in
somatic embryos of Camellia sinensis L. Using degenerate primers with cDNA of Camellia sinensis, a 457 bp GSHS
gene fragment was cloned through polymerase chain reaction. This fragment was used in making ihpRNA. For this it
was cloned in sense at AscI and SwaI and in anti-sense at Bam HI and XbaI restriction sites of pFGC5941 that has
chalcone synthase (Chs) intron between SwaI and BamHI restriction sites. Resultant RNAi construct was used for
C. sinensis somatic embryos transformation through Agrobacterium. After 11, 13 and 15 d of transformation, embryo
GSHS transcript levels and GSH content decreased to a great extent which documented the feasibility of RNAi based
gene silencing in C. sinensis.
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| Date |
2008
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| Type |
Article
PeerReviewed |
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| Format |
application/pdf
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| Identifier |
http://ihbt.csircentral.net/90/1/2008_Transient_RN.pdf
Mohanpuria, P and Rana, N K and Yadav , S K (2008) Transient RNAi based gene silencing of glutathione synthetase reduces glutathione content in Camellia sinensis (L.) O. Kuntze somatic embryos. Biologia Plantarum, 52 (2). pp. 381-384. |
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| Relation |
http://ihbt.csircentral.net/90/
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