Quantification of Picroside-I and Picroside-II in picrorhiza kurroa by HPTLC.
IR@IHBT: CSIR-Institute of Himalayan Bioresource Technology, Palampur
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| Title |
Quantification of Picroside-I and Picroside-II in picrorhiza kurroa by HPTLC.
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| Creator |
Singh, N
Singh , Bikram Gupta , A P Kaul , V K |
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| Subject |
Natural Product Chemistry
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| Description |
A new high performance thin layer chromatography (HPTLC) method for
the simultaneous quantification of picroside-I and picroside-II in P. kurroa is
described. Separation of picroside-I and picroside-II was achieved by mobile phase
of CHCl3:MeOH (82:18, v/v) on precoated silica gel 60 F254 aluminum plate. The
densitometric determination of picrosides was carried out at 290 nm, in absorptionreflection
mode. The calibration curves were linear in the range of (2–5mg). The
method is simple, specific, rapid, and reliable for simultaneous determination of P-I
and P-II in P. kurroa. The proposed method was applied for accurate quantification
of large number of samples collected from different altitudes of western Himalaya.
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| Publisher |
Taylor & Francis Inc , 325 Chestnut St, Suite 800, Philadelphia, PA 19106 USA
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| Date |
2005
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| Type |
Article
PeerReviewed |
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| Format |
application/pdf
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| Identifier |
http://ihbt.csircentral.net/217/1/JLCRT1.pdf
Singh, N and Singh , Bikram and Gupta , A P and Kaul , V K (2005) Quantification of Picroside-I and Picroside-II in picrorhiza kurroa by HPTLC. Journal of Chromatography and Related Technologies, 28 (11). pp. 1679-1691. ISSN 1082-6076 |
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| Relation |
http://ihbt.csircentral.net/217/
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