Screening for viruses infecting chrysanthemum cultivars in India.
IR@IHBT: CSIR-Institute of Himalayan Bioresource Technology, Palampur
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| Title |
Screening for viruses infecting chrysanthemum cultivars in India.
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| Creator |
Verma, N
Mehra, A Singh, L Hallan, Vipin Singh, A K Jabeen, N Singh, M K Raja, Ram Zaidi, A A |
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| Subject |
Plant sciences
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| Description |
Screening of chrysanthemum cultivars was carried out using enzyme linked immunosorbent assay (ELISA), northern hybridization and reverse transcription polymerase chain reaction (RT-PCR) for Chrysanthemum B (CVB), Cucumber mosaic (CMV), Tomato aspermy (TAV), tospo- and potyviruses. By hybridization and RT-PCR, 48.7%, 42.5%, 26.2%, 12.5% and 6.25% cultivars were found to be infected with CVB, CMV, TAV, tospo- and potyviruses, respectively out of 80 cultivars tested. CVB was the most prevalent virus while infections by poty- and tospoviruses were found less frequently. The percentage of the single virus infection in chrysanthemum cultivars under study was the highest (35%) followed by double (26.25%), triple (8.75%) and for tetra and pentavirus infection it was 2.5%. Nucleic acid based detection techniques were found to be more reliable and sensitive than ELISA.
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| Publisher |
Elsevier Science BV, PB No.211, 1000 AE Amsterdam, The Netherlands.
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| Date |
2007
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| Type |
Article
PeerReviewed |
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| Format |
application/pdf
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| Identifier |
http://ihbt.csircentral.net/185/1/chrysanthemum.pdf
Verma, N and Mehra, A and Singh, L and Hallan, Vipin and Singh, A K and Jabeen, N and Singh, M K and Raja, Ram and Zaidi, A A (2007) Screening for viruses infecting chrysanthemum cultivars in India. Scientia Horticulture, 111 (3). pp. 260-265. ISSN 0304-4238 |
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| Relation |
http://ihbt.csircentral.net/185/
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