Simultaneous determination of quercetin, rutin and coumaric acid in flowers of Rhododendron arboreum by HPTLC.
IR@IHBT: CSIR-Institute of Himalayan Bioresource Technology, Palampur
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| Title |
Simultaneous determination of quercetin, rutin and coumaric acid in flowers of Rhododendron arboreum by HPTLC.
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| Creator |
Swaroop, A
Gupta , A P Sinha, Arun K |
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| Subject |
Chemical Engineering
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| Description |
A simple and fast method was developed for simultaneous quantitative determination of three biologically active phenolic compounds i.e. quercetin, rutin and coumaric acid in flowers of Rhododendron orboreum using high-performance thin-layer chromatography (HPTLC). The separation was performed on TLC aluminium plates precoated with silica gel RP-18 F-254S. Good separation was achieved in the mobile phase of methanol-water-formic acid (40:57:3, v/v/v) and densitometric determination of these compounds was carried out at 280 nm in reflectance/absorbance mode. The linear regression data for the calibration plots showed a good linear relationship with r = 0.9971, 0.9953 and 0.9960 quercetin, rutin and coumaric acid, respectively. Accuracy of the method was checked by recovery study conducted at two different levels with the average recovery of 99.90%, 99.02% and 99.16% for quercetin, rutin and cournaric acid, respectively. The present method is being reported first time and may be used for routine quality control of the flowers of R. arboreum.
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| Publisher |
Vieweg, Abraham-Lincoln-Strabe 46, Postfach 15 47, D-65005 Wiesbaden, Germany
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| Date |
2005
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| Type |
Article
PeerReviewed |
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| Format |
application/pdf
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| Identifier |
http://ihbt.csircentral.net/184/1/chrom2.pdf
Swaroop, A and Gupta , A P and Sinha, Arun K (2005) Simultaneous determination of quercetin, rutin and coumaric acid in flowers of Rhododendron arboreum by HPTLC. Chromatographia, 62 (11-12). pp. 649-652. ISSN 0009-5893 |
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| Relation |
http://ihbt.csircentral.net/184/
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