Multiplex PCR and genome analysis of Carnation mottle virus Indian isolate.
IR@IHBT: CSIR-Institute of Himalayan Bioresource Technology, Palampur
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| Title |
Multiplex PCR and genome analysis of Carnation mottle virus Indian isolate.
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| Creator |
Raikhy, G
Hallan, Vipin Kulshrestha, S Raja, Ram Zaidi, A A |
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| Subject |
Plant sciences
Plant viruses |
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| Description |
The complete nucleotide sequence of an Indian isolate of
Carnation mottle virus (CarMV) was determined to be
4005 bp in length, 2 bp longer than previous reports.
The viral genome was amplified by RT–PCR using
primers covering the entire genome length. Coat protein
(CP) and movement protein (MP) genes were also
amplified by multiplex RT–PCR from annual and
perennial carnations. Identity of the amplified products
was verified by sequencing. CP and MP genes of four
Indian isolates were compared with the available sequences
in the GenBank database. CP gene of annual
and perennial carnations showed high similarity (95–
100%) compared to isolates reported from different
parts of the world. MP genes (p7 and p9) showed 95–
98% nucleotide similarity while at the amino acid
level similarity, of 86–98% for p7 and 92–98% for p9
respectively, was obtained. Recombination detection
analysis indicated recombination in an isolate from
annual carnation and division of CarMV isolates into
six groups on the basis of recombination analysis.
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| Publisher |
Current Science Association, C V Raman Avenue, PO Box 8005, Bangalore 560 080, India .
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| Date |
2006
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| Type |
Article
PeerReviewed |
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| Format |
application/pdf
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| Identifier |
http://ihbt.csircentral.net/178/1/CarMV.pdf
Raikhy, G and Hallan, Vipin and Kulshrestha, S and Raja, Ram and Zaidi, A A (2006) Multiplex PCR and genome analysis of Carnation mottle virus Indian isolate. Current Science, 90 (1). pp. 74-82. ISSN 0011-3891 |
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| Relation |
http://ihbt.csircentral.net/178/
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