Simultaneous Determination of Ten Sugars in Tinospora cordifolia by Ultrasonic Assisted Extraction and LC-ELSD
IR@IHBT: CSIR-Institute of Himalayan Bioresource Technology, Palampur
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| Title |
Simultaneous Determination of Ten Sugars in Tinospora cordifolia by Ultrasonic Assisted Extraction and LC-ELSD
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| Creator |
Sharma, U
Bhandari, P Kumar, Neeraj Singh , Bikram |
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| Subject |
Natural Product Chemistry
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| Description |
Sugars present in medicinal plants are known for protecting and stimulating the immune system against various biological disorders. Tinospora cordifolia is a reputed Indian herb used for immunity enhancing which is mainly attributed to saccharides. In the present study, a
simple, sensitive, and reliable liquid chromatography method based on ultrasonic assisted extraction and evaporative light scattering detection was developed for simultaneous determination of ten sugars comprising of monosaccharides (L-(+)-rhamnose, D-(+)-xylose,
D-(-)-arabinose, b-D-(+)-glucose), disaccharides (sucrose, D-(+)-cellobiose, a-lactose), alditols
(xylitol, D-(+)-mannitol) and a polyalcohol (myo-inositol) in T. cordifolia. The separation was
achieved on Zorbax-NH2 column (250 mm 9 4.6 mm, 5 lm) in gradient elution of acetonitrile:
water as mobile phase with flow rate of 0.5 mL min-1. The drift tube temperature and
nitrogen flow-rate were optimized at 70 �C and 2.0 standard litres per minute, respectively.
The method was validated for linearity, accuracy, precision, limits of detection and quantification.
The calibration equation revealed a good linear relationship (r2 = 0.959–0.999).
The sufficient recovery was observed in the range of 94.1–99.9%. The method showed good
reproducibility with intra- and inter-day precision of <0.99 and 0.97% (RSD), respectively.
The detection and quantification limits for the compounds were in the range of 8.32–44.29 and 25.23–134.20 lg mL-1, respectively.
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| Publisher |
Springer Heidelberg , Tiergartenstrasse 17, D-69121 Heidelberg, Germany
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| Date |
2010
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| Type |
Article
PeerReviewed |
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| Format |
application/pdf
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| Identifier |
http://ihbt.csircentral.net/130/1/2010_Simultaneous.pdf
Sharma, U and Bhandari, P and Kumar, Neeraj and Singh , Bikram (2010) Simultaneous Determination of Ten Sugars in Tinospora cordifolia by Ultrasonic Assisted Extraction and LC-ELSD. Chromatographia, 71 (7-8). pp. 633-638. ISSN 0009-5893 |
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| Relation |
http://ihbt.csircentral.net/130/
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