Studies on leaf spot disease of Withania somnifera and its impact on secondary metabolites.
IR@IHBT: CSIR-Institute of Himalayan Bioresource Technology, Palampur
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| Title |
Studies on leaf spot disease of Withania somnifera and its
impact on secondary metabolites.
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| Creator |
Pati, P K
Sharma, Madhu Sharma, M Salar, R K Sharma, A Gupta, A P Singh, B |
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| Subject |
Nutraceuticals
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| Description |
During an investigation of the disease profile of Withania somnifera, it was observed that leaf spot is the
most prevalent disease. Repeated isolations from infected
leaf tissues and pathogenicity tests showed the association
of fungal pathogen identifi ed as Alternaria alternata (Fr.)
Keissler. Scanning electron microscopy showed various
histological changes in the leaf tissues of infected plants.
A decrease in total content of reducing sugars (20%) and
chlorophyll (26.5%) was observed in diseased leaves
whereas an increase was noticed in proline (25%), free
amino acids (3%) and proteins (74.3%). High performance
thin layer chromatography (HPTLC) analysis of secondary
metabolites viz. withanolides, withaferin-A and total
alkaloids of the diseased leaves vis-à-vis control revealed
reduction in withaferin-A and withanolides contents by
15.4% and 76.3% respectively, in contrast to an increase in
total alkaloids by 49.3%, information hitherto unreported in
W. somnifera.
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| Publisher |
Springer, 233 SPRING ST, NEW YORK, NY 10013 USA
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| Date |
2008
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| Type |
Article
PeerReviewed |
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| Format |
application/pdf
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| Identifier |
http://ihbt.csircentral.net/129/1/2008_Studies.pdf
Pati, P K and Sharma, Madhu and Sharma, M and Salar, R K and Sharma, A and Gupta, A P and Singh, B (2008) Studies on leaf spot disease of Withania somnifera and its impact on secondary metabolites. Indian Journal of Microbiology, 48 (4). pp. 432-437. ISSN 0046-8991 |
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| Relation |
http://ihbt.csircentral.net/129/
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