Improved HPTLC Method for Determination of Curcuminoids from Curcuma longa
IR@IHBT: CSIR-Institute of Himalayan Bioresource Technology, Palampur
View Archive Info| Field | Value | |
| Title |
Improved HPTLC Method for Determination of Curcuminoids
from Curcuma longa
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| Creator |
Pathania, V
Gupta , A P Singh , Bikram |
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| Subject |
Natural Product Chemistry
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| Description |
A rapid, improved, simple, and precise high performance thin layer chromatography
method has been developed for the quantification of curcuminoids in
Curcuma longa. Separation of curcuminoids was achieved by employing a mobile
phase consisting of chloroform-methanol (98 : 2 v/v) on precoated HPTLC LiChrosphere
aluminium plates Si 60F254. Densitometry analysis was carried out at 366 nm
in adsorption reflection mode. The method was validated for precision and recovery.
Use of LiChrosphere HPTLC plates gave better resolution, reproducibility, good selectivity
and compact band as compared to other plates. Plant material collected from midhills
showed higher contents of curcuminoids.
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| Publisher |
Taylor & Francis, Inc., 325 Chestnut Street, Suite 800, Philadelphia, PA 19106
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| Date |
2006
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| Type |
Article
PeerReviewed |
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| Format |
application/pdf
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| Identifier |
http://ihbt.csircentral.net/109/1/2006_Improved_HPTLC.pdf
Pathania, V and Gupta , A P and Singh , Bikram (2006) Improved HPTLC Method for Determination of Curcuminoids from Curcuma longa. Journal of Liquid Chromatography & Related Technologies, 29. pp. 875-885. ISSN 1082-6076 |
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| Relation |
http://ihbt.csircentral.net/109/
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