Osmotin-regulated reserve accumulation and germination in genetically transformed tea somatic embryos: a step towards regulation of stress tolerance and seed recalcitrance
IR@IHBT: CSIR-Institute of Himalayan Bioresource Technology, Palampur
View Archive Info| Field | Value | |
| Title |
Osmotin-regulated reserve accumulation and germination in
genetically transformed tea somatic embryos: a step towards
regulation of stress tolerance and seed recalcitrance
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| Creator |
Bhattacharya, Amita
Saini, U Sharma, P Nagar , P K Ahuja, Paramvir Singh |
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| Subject |
Plant sciences
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| Description |
The osmotin gene was introduced into tea [Camellia
sinensis (L.) O. Kuntze] somatic embryos at the
globular stage of development via microprojectile
bombardment, to determine the effects of this stresstolerance-related gene on the accumulation of storage
reserves and acquisition of desiccation tolerance
during embryo maturation. Changes in total soluble
sugars, starch and proteins in response to osmotin
were estimated in the stably transformed embryos,
using standard biochemical and histochemical
methods, after the globular embryos had matured to
the heart stage of development. These changes also
were compared with two types of controls, comprising
(1) untransformed embryos and (2) embryos that were
bombarded without osmotin DNA. The control
embryos showed poor reserve accumulation and
negligible germination but, in contrast, the embryos
bombarded with the osmotin gene showed a severalfold
increase in storage reserves, normal maturation
and 57–63% germination. When these transformed
embryos were desiccated to 15.5% moisture content,
only 40% germinated, although a further increase in
the accumulation of storage reserves occurred. In
contrast, the control embryos became necrotic and
failed to germinate. This study demonstrates the use of
somatic embryogenesis as a tool for understanding
seed recalcitrance in tea, and suggests that transformation
with stress-tolerance genes can overcome
the developmental problems associated with recalcitrant
seeds.
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| Publisher |
Cambridge University Press , Edinburgh BLDG, Shaftesbury RD, CB2 2RU Cambridge, England
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| Date |
2006
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| Type |
Article
PeerReviewed |
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| Format |
application/pdf
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| Identifier |
http://ihbt.csircentral.net/52/1/2006_Osmotin%2Dregulated.pdf
Bhattacharya, Amita and Saini, U and Sharma, P and Nagar , P K and Ahuja, Paramvir Singh (2006) Osmotin-regulated reserve accumulation and germination in genetically transformed tea somatic embryos: a step towards regulation of stress tolerance and seed recalcitrance. Seed Science Research, 16. pp. 203-211. ISSN 0960-2585 |
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| Relation |
http://ihbt.csircentral.net/52/
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