A Novel Homologue of coronin colocalizes with actin in filament-like structures in Leishmania
IR@CDRI: CSIR-Central Drug Research Institute, Lucknow
View Archive Info| Field | Value | |
| Creator |
Nayak, Ramesh C.
Sahasrabuddhe, Amogh A. Bajpai, V K Gupta, C M |
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| Date |
2008-12-22T13:56:35Z
2008-12-22T13:56:35Z 2005 |
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| Identifier |
Molecular & Biochemical Parasitology,143,152-64(2005)
http://hdl.handle.net/123456789/245 |
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| Description |
The presence of actin in Leishmania has recently been demonstrated, but the functional form of this protein (filamentous actin) has not yet been identified. We report here that the putative coronin homologue identified in the Leishmania genome is invariably associated with the filament-like structures of actin in Leishmania promastigotes. The occurrence of filamentous structures is significantly increased upon over expression of Leishmania coronin as its GFP fusion product in Leishmania cells. However, expression of Leishmania actin or coronin alone in mammalian cells does not result in formation of any filament-like structures of Leishmania actin or association of Leishmania coronin with mammalian filamentous actin, but coexpression of both the proteins in these cells leads to formation of filamentous structures containing Leishmania actin and coronin. The high specificity of Leishmania coronin for Leishmania actin could be attributed to its unique structure as it differs from other coronins not only in the unique region but also in the actin-binding site and leucine zipper motif. These results taken together indicate that Leishmania contains a novel form of coronin which colocalizes with actin in filament-like structures in these cells.
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| Format |
6361981 bytes
application/pdf |
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| Language |
en
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| Relation |
CDRI Communication No 6621
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| Subject |
Actin
Coronin Filament dynamics Leishmania |
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| Title |
A Novel Homologue of coronin colocalizes with actin in filament-like structures in Leishmania
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| Type |
Article
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