Targeted cytosolic delivery of hydrogel nanoparticles into HepG2 cells through engineered Sendai viral envelopes
IR@CDRI: CSIR-Central Drug Research Institute, Lucknow
View Archive Info| Field | Value | |
| Creator |
Jana, S S
Bharali, D J Mani, Prashant Maitra, Amarnath Gupta, C M Sarkar, D P |
|
| Date |
2008-10-23T20:54:15Z
2008-10-23T20:54:15Z 2002 |
|
| Identifier |
FEBS Letters 515, 184- 189
http://hdl.handle.net/123456789/206 |
|
| Description |
Hydrogel nanoparticles of cross-linked polyvinylpyrrolidone (PVP-NP) (35-50 nm in diameter) containing fluoresceinated dextran (FITC-Dx) were encapsulated in reconstituted Sendai viral envelopes containing only the fusion (F) protien (F- virosomes). Incubtion of these loaded F- virosmes with human hepatoblastoma cells (HepG2) in culture cytoplasm, as inferred froom the ability of cells tointernalize FITC-Dx loaded PVP-NP(PVP-NP) in the presence of azide (an inhibitor of the endocytotic process). Introduction of PVP-NP into the HelpG2 cells wasw assured by selctive accumulation of FITC fluorescence in the cytosolic compartment. The structural integrity of the internalzed PVP-NP was also confirmed by fluorescence microscopy and ultracentrifugation analysis. The potential usefulness of PVP-NP -mediated cytosolic release of water soluble drugs boyh in vitro and in vivo has been established for the first time.
|
|
| Format |
3058853 bytes
application/pdf |
|
| Language |
en
|
|
| Subject |
Hydrogel nanoparticle
Sendai virus Virosome Drug delivery Targeted cytosolic delivery Controlled release |
|
| Title |
Targeted cytosolic delivery of hydrogel nanoparticles into HepG2 cells through engineered Sendai viral envelopes
|
|
| Type |
Article
|
|