Expression, purification, crystallization and preliminary X-ray analysis of maleylacetate reductase from Burkholderia sp. strain SJ98.
IR@IMTECH: CSIR-Institute of Microbial Technology, Chandigarh
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| Title |
Expression, purification, crystallization and preliminary X-ray analysis of maleylacetate reductase from Burkholderia sp. strain SJ98.
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| Creator |
Chauhan, Archana
Islam, Zeyaul Jain, R K Karthikeyan, Subramanian |
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| Subject |
QD Chemistry
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| Description |
Maleylacetate reductase (EC 1.3.1.32) is an important enzyme that is involved in the degradation pathway of aromatic compounds and catalyzes the reduction of maleylacetate to 3-oxoadipate. The gene pnpD encoding maleylacetate reductase in Burkholderia sp. strain SJ98 was cloned, expressed in Escherichia coli and purified by affinity chromatography. The enzyme was crystallized in both native and SeMet-derivative forms by the sitting-drop vapour-diffusion method using PEG 3350 as a precipitant at 293 K. The crystals belonged to space group P2(1)2(1)2, with unit-cell parameters a = 72.91, b = 85.94, c = 53.07 A. X-ray diffraction data for the native and SeMet-derivative crystal were collected to 2.7 and 2.9 A resolution, respectively.
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| Publisher |
Wiley
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| Date |
2009-12-01
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| Type |
Article
PeerReviewed |
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| Format |
application/pdf
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| Identifier |
http://crdd.osdd.net/open/538/1/Karthi%2Dacta10.pdf
Chauhan, Archana and Islam, Zeyaul and Jain, R K and Karthikeyan, Subramanian (2009) Expression, purification, crystallization and preliminary X-ray analysis of maleylacetate reductase from Burkholderia sp. strain SJ98. Acta crystallographica. Section F, Structural biology and crystallization communications, 65 (Pt 12). pp. 1313-6. ISSN 1744-3091 |
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| Relation |
http://journals.iucr.org/f/issues/2009/12/00/us5002/us5002.pdf
http://crdd.osdd.net/open/538/ |
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