Efficient ligation and cloning of DNA fragments with 2-bp overhangs.
IR@IMTECH: CSIR-Institute of Microbial Technology, Chandigarh
View Archive Info| Field | Value | |
| Title |
Efficient ligation and cloning of DNA fragments with 2-bp overhangs.
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| Creator |
Ranjan, Rajiv Kumar
Rajagopal, Kammara |
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| Subject |
QD Chemistry
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| Description |
Various methods of ligation are currently available and routinely used by molecular biologists, such as blunt end ligation, cohesive end (two and four overhangs), and ligation of Taq polymerase-derived products. However, there is no efficient method for the cloning of DNA fragments with 2-bp overhangs. We present a simple method for the efficient ligation of DNA fragments with 2-bp overhanging ends, ranging in size from 0.7 to 2.5 kbp. Our method involves the initial heating and flash freezing of the vector-insert DNA mix, and a subsequent unique ligation reaction. This method provides a new molecular biology tool for researchers.
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| Publisher |
Elsevier Science
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| Date |
2010-07-01
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| Type |
Article
PeerReviewed |
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| Relation |
http://www.sciencedirect.com/science/article/pii/S0003269710001880
http://crdd.osdd.net/open/526/ |
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| Identifier |
Ranjan, Rajiv Kumar and Rajagopal, Kammara (2010) Efficient ligation and cloning of DNA fragments with 2-bp overhangs. Analytical biochemistry, 402 (1). pp. 91-2. ISSN 1096-0309
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