Intracellular Replication of Choleraphage Ê92
IR@IICB: CSIR-Indian Institute of Chemical Biology, Kolkata
View Archive Info| Field | Value | |
| Title |
Intracellular Replication of
Choleraphage Ê92
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| Creator |
Barman, Subrata
Majumdar, Sabita |
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| Subject |
Infectious Diseases and Immunology
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| Description |
The broad host range choleraphage Ê92 contains a linear
double-stranded DNA molecule of 68 kilobase (kb) pairs
with 3) overhang cohesive ends. Following infection with
phage Ê92, the host macromolecular synthesis is shut off
within the first 5 min, and synthesis of phage-specific
DNA is detectable after 7 min of infection. At early times
during infection, phage DNA is replicated in circular form
and the synthesis is membrane associated. The closed
circular form serves as the precursor for the synthesis of
the mature phage DNA which is eventually packaged
into the phage head. Pulse labeling of ultraviolet-irradiated
infected cells at different times during infection
has allowed identification of about 30 phage-specific
proteins of which 6 are structural proteins. These proteins
appear during the infection cycle in two distinct
phases, early and late. Eighteen early and 12 late proteins
have been identified.
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| Publisher |
Karger
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| Date |
1999
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| Type |
Article
PeerReviewed |
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| Format |
application/pdf
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| Identifier |
http://www.eprints.iicb.res.in/675/1/INTERVIROLOGY_42_(4)_238%2D246_;1999[40].pdf
Barman, Subrata and Majumdar, Sabita (1999) Intracellular Replication of Choleraphage Ê92. Intervirology, 42 (4). pp. 238-246. |
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| Relation |
http://dx.doi.org/10.1159/000024983
http://www.eprints.iicb.res.in/675/ |
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