Accelerated Publication Role of superoxide dismutase in survival of Leishmania within the macrophage
IR@IICB: CSIR-Indian Institute of Chemical Biology, Kolkata
View Archive InfoField | Value | |
Title |
Accelerated Publication
Role of superoxide dismutase in survival of Leishmania within
the macrophage
|
|
Creator |
Ghosh, Sanjay
Goswami, Srikanta Adhya, Samit |
|
Subject |
Molecular & Human Genetics
|
|
Description |
Intracellular parasitic protozoans of the genus Leishmania depend
for their survival on the elaboration of enzymic and other
mechanisms for evading toxic free-radical damage in¯icted by
their phagocytic macrophage host. One such mechanism may
involve superoxide dismutase (SOD), which detoxi®es reactive
superoxide radicals produced by activated macrophages, but the
role of this enzyme in parasite survival has not yet been
demonstrated. We have cloned a SOD gene from L. tropica and
generated SOD-de®cient parasites by expressing the corresponding antisense RNA from an episomal vector. Such parasites have
enhanced sensitivity to menadione and hydrogen peroxide in
axenic culture, and a markedly reduced survival in mouse
macrophages. These results indicate that SOD is a major
determinant of intracellular survival of Leishmania.
|
|
Publisher |
Portland Press
|
|
Date |
2003
|
|
Type |
Article
PeerReviewed |
|
Format |
application/pdf
|
|
Identifier |
http://www.eprints.iicb.res.in/593/1/BIOCHEMICAL_JOURNAL%2C369%2C_447%2D452[82].pdf
Ghosh, Sanjay and Goswami, Srikanta and Adhya, Samit (2003) Accelerated Publication Role of superoxide dismutase in survival of Leishmania within the macrophage. Biochemical Journal, 369. pp. 447-452. ISSN 0264-6021 |
|
Relation |
http://dx.doi.org/10.1042/BJ20021684
http://www.eprints.iicb.res.in/593/ |
|