Role of sulfhydryl groups in band 3 in the inhibition of phosphate transport across erythrocyte membrane in visceral leishmaniasis
IR@IICB: CSIR-Indian Institute of Chemical Biology, Kolkata
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| Title |
Role of sulfhydryl groups in band 3 in the inhibition of phosphate
transport across erythrocyte membrane in visceral leishmaniasis
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| Creator |
Saha Roy, Sudipa
Sen, Gargi Biswas, Tuli |
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| Subject |
Cell Biology & Physiology
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| Description |
Membrane destabilization in erythrocytes plays an important role in the premature hemolysis and development of anemia during
visceral leishmaniasis (VL). Marked degradation of the anion channel protein band 3 is likely to allow modulation of anion flux
across the red cell membrane in infected animals. The present study describes the effect of structural modification of band 3 on
phosphate transport in VL using 31P NMR. The result showed progressive decrease in the rate and extent of phosphate transport
during the post-infection period. Interdependence between the intracellular ionic levels seems to be a determining factor in the
regulation of anion transport across the erythrocyte membrane in control and infected conditions. Infection-induced alteration
in band 3 made the active sites of transport more susceptible to binding with amino reactive agents. Inhibition of transport by
oxidation of band 3 and subsequent reversal by reduction using dithiothreitol suggests the contribution of sulfhydryl group in
the regulation of anion exchange across the membrane. Quantitation of sulfhydryl groups in the anion channel protein showed
the inhibition to be closely related to the decrease of sulfhydryl groups in the infected hamsters. Downregulation of phosphate
transport during leishmanial infection may be ascribed to the sulfhydryl modification of band 3 resulting in the impaired functioning
of this protein under the diseased condition.
� 2005 Elsevier Inc. All rights reserved.
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| Publisher |
Elsevier
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| Date |
2005
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| Type |
Article
PeerReviewed |
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| Format |
application/pdf
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| Identifier |
http://www.eprints.iicb.res.in/497/1/ARCHIVES_OF_BIOCHEMISTRY_AND_BIOPHYSICS_436(_1)_121%2D127;21005[74].pdf
Saha Roy, Sudipa and Sen, Gargi and Biswas, Tuli (2005) Role of sulfhydryl groups in band 3 in the inhibition of phosphate transport across erythrocyte membrane in visceral leishmaniasis. Archives of Biochemistry and Biophysics, 436. pp. 121-127. |
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| Relation |
http://dx.doi.org/10.1016/j.abb.2005.01.015
http://www.eprints.iicb.res.in/497/ |
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