Interleukin-1<img src='/image/spc_char/beta.gif' border=0>-induced iNOS expression in human lung carcinoma A549 cells: Involvement of STAT and MAPK pathways
IR@NISCAIR: CSIR-NISCAIR, New Delhi - ONLINE PERIODICALS REPOSITORY (NOPR)
View Archive Info| Field | Value | |
| Creator |
Ravichandran, Kameswaran
Tyagi, Alpna Deep, Gagan Agarwal, Chapla Agarwal, Rajesh |
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| Date |
2011-11-01T04:59:16Z
2011-11-01T04:59:16Z 2011-11 |
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| Identifier |
0975-1009 (Online); 0019-5189 (Print)
http://hdl.handle.net/123456789/13001 |
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| Description |
840-847
For understanding of signaling molecules important in lung cancer growth and progression, IL-1<img src='/image/spc_char/beta.gif' border=0> effect was analyzed on iNOS expression and key signaling molecules in human lung carcinoma A549 cells and established the role of specific signaling molecules by using specific chemical inhibitors.<b style=""> </b>IL-1<img src='/image/spc_char/beta.gif' border=0> exposure (10 ng/ml) induced strong iNOS expression in serum starved A549 cells. Detailed molecular analyses showed that IL-1<img src='/image/spc_char/beta.gif' border=0> increased expression of phosphorylated STAT1 (Tyr701 and Ser727) and STAT3 (Tyr705 and Ser727) both in total cell lysates and nuclear lysates. Further, IL-1b exposure strongly activated MAPKs (ERK1/2, JNK1/2 and p38) and Akt as well as increased nuclear levels of NF-κB and HIF-1<img src='/image/spc_char/alpha.gif' border=0> in A549 cells. Use of specific chemical inhibitors for JAK1 kinase (piceatannol), JAK2 kinase (AG-490), MEK1/2 (PD98059) and JNK1/2 (SP600125) revealed that IL-1<img src='/image/spc_char/beta.gif' border=0>-induced iNOS expression involved signaling pathways in addition to JAK-STAT and ERK1/2-JNK1/2 activation. Overall, these results suggested that instead of specific pharmacological inhibitors, use of chemopreventive agents with broad spectrum efficacy to inhibit IL-1<img src='/image/spc_char/beta.gif' border=0>-induced signaling cascades and iNOS expression would be a better strategy towards lung cancer prevention and/or treatment. |
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| Language |
en_US
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| Publisher |
NISCAIR-CSIR, India
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| Rights |
<img src='http://nopr.niscair.res.in/image/cc-license-sml.png'> <a href='http://creativecommons.org/licenses/by-nc-nd/2.5/in' target='_blank'>CC Attribution-Noncommercial-No Derivative Works 2.5 India</a>
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| Source |
IJEB Vol.49(11) [November 2011]
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| Subject |
Human lung carcinoma
iNOS Interleukin-1<img src='/image/spc_char/beta.gif' border=0> MAPK STAT |
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| Title |
Interleukin-1<img src='/image/spc_char/beta.gif' border=0>-induced iNOS expression in human lung carcinoma A549 cells: Involvement of STAT and MAPK pathways
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| Type |
Article
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