Assessing role of Cra in regulation of <i>prpB </i>and <i>yahA </i>genes of <i>Escherichia coli in vivo </i>using <i>lacZ </i>transcriptional fusions
IR@NISCAIR: CSIR-NISCAIR, New Delhi - ONLINE PERIODICALS REPOSITORY (NOPR)
View Archive Info| Field | Value | |
| Creator |
Ponmani, Thangaraj
Munavar, M Hussain |
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| Date |
2011-10-31T10:14:05Z
2011-10-31T10:14:05Z 2011-11 |
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| Identifier |
0975-1084 (Online); 0022-4456 (Print)
http://hdl.handle.net/123456789/12964 |
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| Description |
938-944
This study presents <i>in vivo </i>role of Cra (Catabolite repressor activator) protein in regulation of two selected genes (<i>prpB </i>& <i>yahA</i>) of <i>Escherichia coli</i>. Gene <i>prpB </i>bears Cra binding site and <i>yahA </i>region binds to Cra <i>in vitro</i>. Therefore, in pBR322 background, p<i>prpB-lacZ </i>and p<i>yahA-lacZ </i>transcriptional fusions were generated and studied expression of b-galactosidase from both fusions in <i>cra</i><i>+ </i>and <i>cra¯ </i>genetic backgrounds of <i>E. coli</i>. Results that both the promoters could possibly be up-regulated by Cra <i>in vivo </i>in <i>E. coli </i>were discussed in relation to transcription control by accessory transcription factor(s). |
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| Language |
en_US
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| Publisher |
NISCAIR-CSIR, India
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| Rights |
<img src='http://nopr.niscair.res.in/image/cc-license-sml.png'> <a href='http://creativecommons.org/licenses/by-nc-nd/2.5/in' target='_blank'>CC Attribution-Noncommercial-No Derivative Works 2.5 India</a>
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| Source |
JSIR Vol.70(11) [November 2011]
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| Subject |
Cra
<i>lacZ</i> <i>prpB</i> <i>yahA</i> Transcriptional fusion |
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| Title |
Assessing role of Cra in regulation of <i>prpB </i>and <i>yahA </i>genes of <i>Escherichia coli in vivo </i>using <i>lacZ </i>transcriptional fusions
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| Type |
Article
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