Purification and characterization of a low molecular weight multifunctional cytotoxic phospholipase A(2) from Russell's viper venom
Metadata of CSIR Papers
View Archive Info| Field | Value | |
| Title |
Purification and characterization of a low molecular weight multifunctional cytotoxic phospholipase A(2) from Russell's viper venom
|
|
| Creator |
Maity, G
Mandal, S Chatterjee, A Bhattacharyya, D |
|
| Subject |
Biochemical Research Methods; Chemistry, Analytical
|
|
| Description |
A basic toxin from Russell's viper venom of 7.2 kDa (RVV-7) has been purified to homogeneity after partial unfolding by 4 M urea followed by filtration through Centricon-30 membrane. Its N-terminal sequence showed strong homology with snake venom cytotoxins. Cytotoxic activity of RVV-7 has been demonstrated with B16F10 melanoma cells. PLA(2) activity was observed in cytotoxin (CX3) from Naja kauthia bearing sequence homology with RVV-7. Phospholipase A(2) and trypsin inhibitory activities were also observed with RVV-7. Chemical modification and inhibition studies suggested independent functional sites for these activities. A qualitative assessment of tumor growth inhibition by RVV-7 has been made. (c) 2006 Published by Elsevier B.V.
|
|
| Publisher |
ELSEVIER SCIENCE BVAMSTERDAMPO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS
|
|
| Date |
2011-09-20T12:12:40Z
2011-09-20T12:12:40Z 2007 |
|
| Type |
Proceedings Paper
|
|
| Identifier |
JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES
1570-0232 http://hdl.handle.net/123456789/14375 |
|
| Language |
English
|
|