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Human placental protein/peptides stimulate melanin synthesis by enhancing tyrosinase gene expression

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Title	Human placental protein/peptides stimulate melanin synthesis by enhancing tyrosinase gene expression

Creator	Sarkar, C Singh, SK Mandal, SK Saha, B Bera, R Ratha, J Datta, PK Bhadra, R

Subject	Cell Biology

Description	Placental protein/peptides as biological response modifier are well documented, but not much known about melanogenesis. We possibly for the first time, demonstrated melanogenesis in B16F10 mouse melanoma by a placental protein/peptide fraction (PPPF) prepared from a hydroalcoholic extract of fresh term human placenta. This study described the effect of PPPF on the induction of tyrosinase; the key enzyme of melanogenesis to investigate the basis of PPPF induced pigmentation in primary melanocyte and B16F10 melanoma. Tyrosinase induction by PPPF in B16F10 cells was found dose- and time dependent at the level of activity. Tyrosinase, at the level of transcription and protein expression when assessed by RT-PCR and Western blot analyses found to have considerable induction over untreated control. PPPF led to enhanced activation of tyrosinase promoter resulting higher transcription thus substantiating the role of PPPF as a stimulator of melanogenesis. Actinomycin D, the transcriptional inhibitor of protein synthesis, blocked the stimulatory action of PPPF since the induction of tyrosinase and melanin was markedly reduced in presence of this inhibitor. Thus the results suggested that PPPF mediated increase in tyrosinase expression occurred through transcriptional upregulation to stimulate melanogenesis in B16F10 cells and in primary melanocyte also.

Publisher	SPRINGERDORDRECHTVAN GODEWIJCKSTRAAT 30, 3311 GZ DORDRECHT, NETHERLANDS

Date	2011-09-20T12:12:13Z 2011-09-20T12:12:13Z 2006

Type	Article

Identifier	MOLECULAR AND CELLULAR BIOCHEMISTRY 0300-8177 http://hdl.handle.net/123456789/14173

Language	English