Production and purification of a bioactive substance inhibiting multiple drug resistant bacteria and human leukemia cells from a salt-tolerant marine Actinobacterium sp isolated from the Bay of Bengal
Metadata of CSIR Papers
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| Title |
Production and purification of a bioactive substance inhibiting multiple drug resistant bacteria and human leukemia cells from a salt-tolerant marine Actinobacterium sp isolated from the Bay of Bengal
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| Creator |
Saha, M
Jaisankar, P Das, S Sarkar, KK Roy, S Besra, SE Vedasiromani, JR Ghosh, D Sana, B Mukherjee, J |
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| Subject |
Biotechnology & Applied Microbiology
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| Description |
Four marine actinobacteria tolerant to 200 g NaCl l(-1) were screened for antibacterial activity against eight patient-derived multiple drug resistant (MDR) bacteria. The active compound (MW 300.2, predicted molecular formula C20H28O2) from an actinobacterium, was inhibitory to three Gram-positive and three Gram-negative MDR bacteria, seven non-clinical Gram-positive, four Gram-negative bacteria and five fungi (MIC: 3.5-4.0 mu g ml(-1)). Also, 54% of human leukemia (HL-60) cells were killed by the compound at 0.05 mu g ml(-1). Bioreactor production demonstrated unusual primary metabolite kinetics. Molecular phylogenetic analysis showed this typical intertidal inhabitant to be a member of the Streptomyces genus and distinct from other salt-tolerant actinobacteria. As no compound was found to match the properties in several electronic databases, our screening strategy should increase the possibility of discovering bioactive molecules from rare actinobacteria.
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| Publisher |
SPRINGERDORDRECHTVAN GODEWIJCKSTRAAT 30, 3311 GZ DORDRECHT, NETHERLANDS
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| Date |
2011-09-20T12:12:12Z
2011-09-20T12:12:12Z 2006 |
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| Type |
Article
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| Identifier |
BIOTECHNOLOGY LETTERS
0141-5492 http://hdl.handle.net/123456789/14166 |
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| Language |
English
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