The alpha-subunit of Leishmania F-1 ATP synthase hydrolyzes ATP in presence of tRNA
Metadata of CSIR Papers
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| Title |
The alpha-subunit of Leishmania F-1 ATP synthase hydrolyzes ATP in presence of tRNA
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| Creator |
Goswami, S
Adhya, S |
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| Subject |
Biochemistry & Molecular Biology
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| Description |
Import of tRNAs into the mitochondria of the kinetoplastid protozoon Leishmania requires the tRNA-dependent hydrolysis of ATP leading to the generation of membrane potential through the pumping of protons. Subunit RIC1 of the inner membrane RNA import complex is a bi-functional protein that is identical to the alpha-subunit of F1F0 ATP synthase and specifically binds to a subset (Type I) of importable tRNAs. We show that recombinant, purified RIC1 is a Type I tRNA-dependent ATP hydrolase. The activity was insensitive to oligomycin, sensitive to mutations within the import signal of the tRNA, and required the cooperative interaction between the ATP-binding and C-terminal domains of RIC1. The ATPase activity of the intact complex was inhibited by anti-RIC1 antibody, while knockdown of RIC1 in Leishmania tropica resulted in deficiency of the tRNA-dependent ATPase activity of the mitochondrial inner membrane. Moreover, RIC1 knockdown extracts failed to generate a membrane potential across reconstituted proteoliposomes, as shown by a rhodamine 123 uptake assay, but activity was restored by adding back purified RIC1. These observations identify RIC1 as a novel form of the F-1 ATP synthase alpha-subunit that acts as the major energy transducer for tRNA import.
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| Publisher |
AMER SOC BIOCHEMISTRY MOLECULAR BIOLOGY INCBETHESDA9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3996 USA
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| Date |
2011-09-20T12:12:11Z
2011-09-20T12:12:11Z 2006 |
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| Type |
Article
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| Identifier |
JOURNAL OF BIOLOGICAL CHEMISTRY
0021-9258 http://hdl.handle.net/123456789/14164 |
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| Language |
English
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