Increased interferon gamma production by peripheral blood mononuclear cells in response to stimulation of overexpressed disease-specific 9-O-acetylated sialoglycoconjugates in children suffering from acute lymphoblastic leukaemia
Metadata of CSIR Papers
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| Title |
Increased interferon gamma production by peripheral blood mononuclear cells in response to stimulation of overexpressed disease-specific 9-O-acetylated sialoglycoconjugates in children suffering from acute lymphoblastic leukaemia
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| Creator |
Ghosh, S
Bandyopadhyay, S Pal, S Das, D Bhattacharya, DK Mandal, C |
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| Subject |
Hematology
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| Description |
Disease-specific over-expression of 9-O-acetylated sialoglycoconjugates (9-O-AcSGs) on peripheral blood mononuclear cells (PBMC) of children with acute lymphoblastic leukaemia (ALL, PBMCALL) has been demonstrated using a lectin, Achatinin-H, with specificity towards 9-O-AcSAalpha2-6GalNAc. This study investigated the contributory role of 9-O-AcSGs induced on PBMCALL. Stimulation of PBMCALL with Achatinin-H through 9-O-AcSGs led to a lymphoproliferative response with a significantly increased interferon-gamma (IFN-gamma) production when compared with unstimulated cells as demonstrated by enzyme-linked immunosorbent assay and mRNA expression. Under identical conditions, PBMCALL ablated of O-acetylations did not respond to such stimulation. In summary, it may be concluded that stimulation of over-expressed 9-O-AcSGs regulate signalling for proliferation, leading to the release of IFN-gamma. Controlled expression of these molecules may be exploited as potential targets for therapy, promising beneficial effects to children with ALL.
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| Publisher |
BLACKWELL PUBLISHING LTDOXFORD9600 GARSINGTON RD, OXFORD OX4 2DG, OXON, ENGLAND
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| Date |
2011-09-20T12:12:00Z
2011-09-20T12:12:00Z 2005 |
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| Type |
Article
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| Identifier |
BRITISH JOURNAL OF HAEMATOLOGY
0007-1048 http://hdl.handle.net/123456789/14075 |
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| Language |
English
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