Metadata of CSIR Papers
View Archive Info| Field | Value | |
| Creator |
Sharma, N
Sharma, UK Gupta, AP Sinha, AK |
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| Subject |
Chemistry; Food Science & Technology
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| Description |
A rapid and sensitive reverse phase high-performance thin-layer chromatographic (RP-HPTLC) method has been developed for the simultaneous determination of four bioactive phenolics, viz. epicatechin (1), syringic acid (2), quercetin-3-O-galactoside (3) and quercitrin (4) in the leaves of Rhododendron arboreum, Rhododendron campanulatum and Rhododendron anthopogon. HPTLC was performed on pre-coated RP-18 F(254S) TLC plates. For achieving good separation, the mobile phase of methanol-5% formic acid in water, 50:50 (v/v) was used and densitometric determination of compounds was carried out at 290 nm in reflection/absorption mode. The method was validated in terms of linearity, precision, accuracy, and sensitivity. The calibration curves were linear in the range of 200-1200 ng for compounds 1,3 and 4 and 400-2400 ng for compound 2. Lower limits of detection obtained for compounds 1-4 were 20, 40, 25 and 25 ng respectively, while the limit of quantification obtained were 50, 115, 75 and 70 ng respectively. The method was found to be reproducible and convenient for quantitative analysis of these compounds in Rhododendron species. (C) 2010 Elsevier Inc. All rights reserved.
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| Publisher |
ACADEMIC PRESS INC ELSEVIER SCIENCESAN DIEGO525 B ST, STE 1900, SAN DIEGO, CA 92101-4495 USA
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| Date |
2011-09-20T12:08:56Z
2011-09-20T12:08:56Z 2010 |
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| Type |
Article
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| Identifier |
JOURNAL OF FOOD COMPOSITION AND ANALYSIS
0889-1575 http://hdl.handle.net/123456789/13570 |
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| Language |
English
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