Spectrophotometric determination of lamuvidine using acidic dye and coupling reagent
IR@NISCAIR: CSIR-NISCAIR, New Delhi - ONLINE PERIODICALS REPOSITORY (NOPR)
View Archive Info| Field | Value | |
| Title |
Spectrophotometric determination of lamuvidine using acidic dye and coupling reagent
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| Creator |
Reddy, M B Rahul
Gurupadayya, B M Kumar, T Anil |
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| Subject |
3-methyl-2-benzothiazolinone
Acidic dye Bromophenol blue Lamivudine hydrazone Spectrophotometric study |
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| Description |
431-438
Two simple, extraction-free spectrophotometric methods (Method-I and Method-II) for the quantitative estimation of lamuvidine (LMV) in bulk drug and pharmaceutical formulations (tablets) have been developed. First method is based on the interaction of LMV with 0.1% methanolic solution of bromophenol blue to form a stable, red-colored, ion-pair complex showing the peak at 595 nm. Second method is based on the oxidation followed by coupling of 3-methyl-2-benzothiazolinone hydrazone with LMV in the presence of ferric chloride to form green-colored chromogen exhibiting absorption maximum at 659 nm. Both the methods follow Beer’s law in the concentration range 1-8 μg mL<sup>-1</sup>. Linear relationships with good correlation coefficients (0.991-0.9967) are found between absorbance and corresponding concentrations of the drug. The reliability and performance of the proposed methods are validated statistically, the recovery ranges from 99.4 ± 0.1% to 99.9 ± 0.3%. The results of analysis for the two methods have been validated statistically and by recovery studies. |
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| Date |
2011-12-24T10:48:50Z
2011-12-24T10:48:50Z 2011-11 |
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| Type |
Article
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| Identifier |
0975-0991 (Online); 0971-457X (Print)
http://hdl.handle.net/123456789/13274 |
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| Language |
en_US
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| Rights |
<img src='http://nopr.niscair.res.in/image/cc-license-sml.png'> <a href='http://creativecommons.org/licenses/by-nc-nd/2.5/in' target='_blank'>CC Attribution-Noncommercial-No Derivative Works 2.5 India</a>
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| Publisher |
NISCAIR-CSIR, India
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| Source |
IJCT Vol.18(6) [November 2011]
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